chemical

(-)-Cytisine

C-130

C-130_10 mg

(-)-Cytisine

Non Antibodies

S. Molecules

Confirmed by amino acid analysis and mass spectrometry

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

(-)-Cytisine is an alkaloid, which occurs naturally in several types of plants as well as in animals feeding on them (see example in reference 1). (-)-Cytisine is a potent and selective agonist of neuronal nAChR2. It acts as a partial agonist of β2-containing nAChR3.EC50s differ for different subunit-composed channels; for recombinant human channels expressed in Xenopus oocytes the following EC50 values were reported: 25 µM, 39 µM, 67 µM, 72 µM, 2.6 µM 0.9 µM and 71 µM for α2/β2, α2/β4, α3/β2, α3/β4, α4/β2, α4/β4 and α7 respectively4.However, in β2-containing channels, the response to acetylcholine (ACh) is largely reduced when applied in combination with (-)-Cytisine5. Therefore, when applied in vivo it exerts a compound effect of both activating certain nAchRs and inhibiting the effect of ACh on others. For example, (-)-Cytisine was found to have antidepressant-like effects in several animal models of antidepressant efficacy. This was probably achieved by inhibiting the effect of ACh on α2/β4 channel in the basolateral amygdala6.In another study, the effects of (-)-Cytisine were compared to nicotine. Rats were trained to discriminate nicotine from saline and it was confirmed that (-)-Cytisine had a nicotine-like discriminative effect, but it was much less potent than nicotine itself7.

Alomone Labs

nAChR

(-)-Cytisine (#C-130) is a highly pure, synthetic, and biologically active compound.

A nAChR Agonist and a Partial Agonist of β2 Subunit

190.2

Baphitoxine, Sophorine

Synthetic

C11H14N2O.

40 nM - 1 μM.

(-)-Cytisine is a potent and selective agonist of neuronal nAChR. It acts as a partial agonist at β2-containing nAChR1,2.

Any aqueous buffer (up to 100mM stock). Centrifuge all product preparations before use (10000 x g 5 min).

Any aqueous buffer (up to 100mM stock). Centrifuge all product preparations before use (10000 x g 5 min).

Up to one week at 4°C or six months at -20°C.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>95%

485-35-8

Lyophilized powder.

Contact Alomone Labs for technical support and product customization

41116134

Yes

(1R,5S)-1,2,3,4,5,6-hexahydro-1,5-methano-8H-pyrido[1,2-a][1,5]diazocin-8-one.