Capsaicin | Alomone Labs C-125 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Capsaicin

catalog :

C-125

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Nagaoka K, Nagashima T, Asaoka N, Yamamoto H, Toda C, Kayanuma G, et al. Striatal TRPV1 activation by acetaminophen ameliorates dopamine D2 receptor antagonist-induced orofacial dyskinesia. JCI Insight. 2021;6: pubmed publisher
Lee M, Nahorski M, Hockley J, Lu V, Ison G, Pattison L, et al. Human Labor Pain Is Influenced by the Voltage-Gated Potassium Channel KV6.4 Subunit. Cell Rep. 2020;32:107941 pubmed publisher
Martin C, Stoffer C, Mohammadi M, Hugo J, Leipold E, Oehler B, et al. NaV1.9 Potentiates Oxidized Phospholipid-Induced TRP Responses Only under Inflammatory Conditions. Front Mol Neurosci. 2018;11:7 pubmed publisher
Silva M, Martins D, Charrua A, Piscitelli F, Tavares I, Morgado C, et al. Endovanilloid control of pain modulation by the rostroventromedial medulla in an animal model of diabetic neuropathy. Neuropharmacology. 2016;107:49-57 pubmed publisher
Jendryke T, Prochazkova M, Hall B, Nordmann G, Schladt M, Milenkovic V, et al. TRPV1 function is modulated by Cdk5-mediated phosphorylation: insights into the molecular mechanism of nociception. Sci Rep. 2016;6:22007 pubmed publisher

image

image 1 :

Alomone Labs Capsaicin induces Ca2+influx via activation of TRPV1 channels expressed in C6 cells. - Cells were loaded with Fluo-3 AM and stimulated withCapsaicin(#C-125). Changes in intracellular Ca2+were detected via changes in Fluo-3 emission following application. The left panel shows normalized fluorescence before and after application at 20 seconds of 0-10 M Capsaicin (as indicated). The right panel shows normalized fluorescence plotted against Capsaicin concentrations.

image 2 :

Peptide (C)KAEEDEILNRSPRNRK corresponding to amino acid residues 572-587 of rat Canx (AccessionP35565). Intracellular C-terminus.

product information

CAT :

C-125

SKU :

C-125_1 g

Product Name :

Capsaicin

Group Type :

Non Antibodies

Product Type :

S. Molecules

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Storage before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

Vanilloid receptor 1 (VR1, TRPV1) is a nonselective cation channel that is activated by heat and acid, as well as vanilloids1,2.Capsaicin is a TRPV1 agonist, which acts directly on TRPV13. Being small and hydrophobic, capsaicin crosses the plasma membrane readily to reach its intracellular ligand-binding site on TRPV1, leading to channel activation and cation permeation4-6.Capsaicin acts directly on TRPV11, with half-maximal effective concentration of 711.9 nM in Xenopus oocytes3 and 0.29 µM in HEK293 cells7.

Supplier :

Alomone Labs

Target :

TRPV1 channels

Long Description :

Capsaicin (#C-125) is a highly pure, Synthetic, and biologically active compound.

Short Description :

A Powerful TRPV1 Agonist

MW :

305.4

Synonyms :

(E)-Capsaicin, CPS

Source :

Synthetic

Molecular formula :

C18H27NO3.

Effective Concentration :

0.1-10 μM.

Activity :

Capsaicin is one of the most powerful chemical agonists of the TRPV1 (transient receptor potential vanilloid) channel. It is a small lipophilic molecule derived from hot chili peppers that acts on sensory neurons by opening TRPV1, a principal transduction channel for nociception1. Capsaicin acts directly on TRPV12, with half-maximal effective concentration of 711.9 nM in Xenopus oocytes3 and 0.29 μM in HEK293 cells4.

Reconstitution and Solubility :

Ethanol. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

Ethanol. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to one week at 4°C or six months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98% (HPLC)

CAS No :

404-86-4

Form :

Lyophilized Powder

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

(E)-N-[(4-Hydroxy-3-methoxyphenyl)methyl]-8-methyl-6-nonenamide.

more info or order :

Alomone Labs product webpage