Capsazepine | Alomone Labs C-120 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Capsazepine

catalog :

C-120

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Fazzari J, Balenko M, Zacal N, Singh G. Identification of capsazepine as a novel inhibitor of system xc- and cancer-induced bone pain. J Pain Res. 2017;10:915-925 pubmed publisher
De Blas G, Darszon A, Ocampo A, Serrano C, Castellano L, Hernández González E, et al. TRPM8, a versatile channel in human sperm. PLoS ONE. 2009;4:e6095 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membrane (lane 1 and 3) mouse brain membrane (lanes 2 and 4) and human brain glioblastoma U87 MG cell line (lanes 5 and 6) lysate: - 125. Anti-LPAR1 (EDG2) (extracellular) Antibody(#ALR-031) (1:400).346. Anti-LPAR1 (EDG2) (extracellular) Antibody preincubated with the negative control antigen.

image 2 :

Alomone LabsCapsazepineinhibits TRPV1 channels expressed in C6 cells. - mTRPV1-C6 cells were loaded with Fluo-3AM. Changes in intracellular Ca2+were detected via changes in Fluo-3 emission following agonist application. 5 minutes pre-incubation with 10 MCapsazepine(#C-120) inhibited 10 MCapsaicin(#C-125)-evoked rise in intracellular Ca2+. Normalized fluorescence before and after application (arrow) of 10 M Capsaicin in cells pre-incubated without (black) or with 10 M Capsazepine (gray).

product information

CAT :

C-120

SKU :

C-120_10 mg

Product Name :

Capsazepine

Group Type :

Non Antibodies

Product Type :

S. Molecules

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Storage before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

Capsazepine is a synthetic, competitive and selective antagonist of TRPV1 (transient receptor potential vanilloid 1)1 and an activator of amiloride-sensitive epithelial Na+ channel gamma (ENaCγ). It also inhibits K+-dependent ATP hydrolysis with no effect on Na+-ATPase2,3.Capsazepine is being used in in vitro and in vivo pharmacological studies as a standard TRPV1 competitive antagonist.Capsazepine exhibits potent inhibition of expression of the iNOS gene in LPS-stimulated murine macrophages through inactivation of NF-κB4.The amiloride-sensitive epithelial Na+ channel (ENaC) regulates Na+ homeostasis into cells and across epithelia5.TRPV1 is a member of a distinct subgroup of transient receptor potential (TRP) ion channels. These channels are highly expressed on primary nociceptors and can be activated by several different stimuli such as heat, acid, certain arachidonic acid derivatives and direct phosphorylation via PKC6.

Supplier :

Alomone Labs

Target :

TRPV1, ENaCγ channels

Long Description :

Capsazepine (#C-120) is a highly pure, synthetic, and biologically active compound.

Short Description :

A Selective TRPV1 Blocker and an Activator of ENaCγ Channels

MW :

376.9

Source :

Synthetic

Molecular formula :

C19H21ClN2O2S.

Effective Concentration :

0.1-100 µM.

Activity :

Capsazepine is a selective antagonist of the vanilloid VR1 receptor (TRPV1) with IC50 of 40 nM, and an activator of the ENaC channels.

Reconstitution and Solubility :

100 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

100 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>97%

CAS No :

138977-28-3

Form :

Lyophilized Powder

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

N-[2-(4-chlorophenyl)ethyl]-7,8-dihydroxy-1,3,4,5-tetrahydro-2-benzazepine-2-carbothioamide.

more info or order :

Alomone Labs product webpage