CyPPA | Alomone Labs C-110 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

CyPPA

catalog :

C-110

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Chang S, Huang W, Mao X, Mack M. Ethanol's Effects on Transient Receptor Potential Channel Expression in Brain Microvascular Endothelial Cells. J Neuroimmune Pharmacol. 2018;13:498-508 pubmed publisher

image

image 1 :

Alomone Labs M8-B hydrochloride inhibits TRPM8 channels expressed in HEK-293 cells. - Dose response curve of TRPM8 inhibition byM8-B hydrochloride(#M-220). Cells were loaded with Calcium-5 dye incubated for 10 min with increasing concentrations of M8-B hydrochloride and activated by 30 nM Icilin. Changes in intracellular Ca2+following agonist application were detected as changes in 485nm/525nm fluorescence (RFU). IC50was determined at 20 nM.

image 2 :

image 3 :

Alomone Labs CyPPA enhances hSK2 channel currents in transiently transfected HEK293T cells. - Cells were stimulated with 100 ?MCyPPA(#C-110) and currents were measured using whole cell voltage-clamp. A. Time course at -20mV showing the effect of 100 ?M CyPPA and its inhibition with 150 nMApamin (#STA-200). B. Representative currents at control conditions (bottom) and following application of 100 ?M CyPPA (top). Currents were elicited by a 150 ms voltage ramp from -120 to 60 mV every 10 seconds from a holding potential of -80 mV (current responses are shown between -120 and -10 mV). Leaks were subtracted offline.

product information

CAT :

C-110

SKU :

C-110_10 mg

Product Name :

CyPPA

Group Type :

Non Antibodies

Product Type :

S. Molecules

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Storage before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

Ca2+-activated potassium channels (KCa) are a group of 6/7-TM ion channels that selectively transport K+ ions across biological membranes. They are broadly classified into three subtypes: SK, IK and BK channels (small, intermediate and big conductance).Small-conductance Ca2+-activated K+ channels (SK channels) underlie the medium duration after hyperpolarization that follows single or trains of action potentials in many types of neurons. This family is composed of KCa2.1 (SK1), -2.2 (SK2), and -2.3 (SK3) isoforms, which are expressed differentially within the central nervous system (CNS)1,2.CyPPA is an activator of small conductance KCa channels that displays selectivity for SK2 and SK3 channels (EC50 values are 5.6 and 14 µM for SK3 and SK2 respectively and efficacy is 90% and 71% for hSK3 and hSK2, respectively)3. CyPPA displays no activity at SK1 and IK (KCa3.1) channels3.CyPPA is a tool for distinguishing SK2 and SK3 from SK1 and IK; no other agents distinguish these channels from one another. Therefore, it enriches the repertoire of tools available to test the hypothesis that SK channels may be targets for future CNS drugs4.CyPPA was found to block TRPM7 channels, demonstrating an overlap between pharmacological compounds acting on TRP and KCa channels5.

Supplier :

Alomone Labs

Target :

KCa2.2, KCa2.3, TRPM7 channels

Long Description :

CyPPA (#C-110) is a highly pure, synthetic, and biologically active compound.

Short Description :

A Selective and Potent Activator of KCa2.2 and KCa2.3 Channels and a Blocker of TRPM7 Channels

MW :

285.4

Source :

Synthetic

Molecular formula :

C16H23N5.

Effective Concentration :

1-100 μM.

Activity :

CyPPA is an activator of small conductance Ca2+-activated K+ channels KCa2.2 (SK2) and KCa2.3 (SK3) with EC50 values of 5.6 and 14 μM for SK3 and SK2 respectively. It displays no activity at SK1 and intermediate conductance KCa (IK) channels1. CyPPA blocks TRPM7 channels2.

Reconstitution and Solubility :

DMSO or ethanol. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

DMSO or ethanol. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98%

CAS No :

73029-73-9

Form :

Lyophilized Powder

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

N-Cyclohexyl-N-[2-(3,5-dimethyl-pyrazol-1-yl)-6-methyl-4-pyrimidinamine.

more info or order :

Alomone Labs product webpage