Calyculin A | Alomone Labs C-100 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Calyculin A

catalog :

C-100

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Fournier C, Zahnreich S, Kraft D, Friedrich T, Voss K, Durante M, et al. The fate of a normal human cell traversed by a single charged particle. Sci Rep. 2012;2:643 pubmed publisher
Mills J, Stone N, Erhardt J, Pittman R. Apoptotic membrane blebbing is regulated by myosin light chain phosphorylation. J Cell Biol. 1998;140:627-36 pubmed

image

image 1 :

Alomone Labs DM-PPP inhibits mGluR1 mediated Ca2+mobilization in U2OS cells. - Dose response of normalized inhibition of human mGluR1 mediated L-Glutamate evoked Ca2+mobilization byDM-PPP(#D-255). IC50was determined at 144 nM. hmGluR1-expressing cells were loaded with Ca2+-sensitive dye incubated with different concentrations of DM-PPP and stimulated by 15 M L-Glutamate (EC80). Changes in intracellular Ca2+following stimulation were detected as changes in maximum relative fluorescence (RLU) using FLIPRTETRA?.

image 2 :

product information

CAT :

C-100

SKU :

C-100_0.1 mg

Product Name :

Calyculin A

Group Type :

Non Antibodies

Product Type :

S. Molecules

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Storage before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

Calyculin A was initially isolated from the marine sponge Discodermia calyx.1 It is a very potent, cell-permeable PP-1 and PP-2 inhibitor (IC50 2nM and 0.5-1nM respectively),2 and extremely specific for PP-1 and PP-2A among other acidic, alkaline and protein phosphatases.2,3

Supplier :

Alomone Labs

Long Description :

Calyculin A (#C-100) is a highly pure, natural, and biologically active compound.

Short Description :

A Potent Inhibitor of Protein Phosphatase 1 and Protein Phosphatase 2

MW :

1009.2

Source :

Natural

Molecular formula :

C50H81N4O15P.

Effective Concentration :

10-100 nM.

Activity :

Calyculin A is a very potent, cell-permeable PP-1 and PP-2 inhibitor (IC50 = 2 nM and 0.5-1 nM respectively)1.

Reconstitution and Solubility :

DMSO, ethanol, acetone, or methanol. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

DMSO, ethanol, acetone, or methanol. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to one week at 4°C or three months at -20°C.Protect from light.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98% (HPLC)

CAS No :

101932-71-2

Form :

Lyophilized Powder

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

N-[(3S)-[4-(1E)-3-[(2R,3R,4R,7S,8S,9R)-2-[(1S,3S,4S,5R, 7E,9E,11E,13Z)-14-Cyano-3,5-dihydroxy-1-methoxy-4,6,8,9 ,13-pentamethyl-7,9,11,13-tetradecatetraenyl]-9-hydroxy -4,4,8-trimethyl-3-(phosphonooxy)-1,6-dioxaspiro[4.5]de c-7-yl]-1-propenyl]-2-oxazoly]butyl]-4-deoxy-4-(dimethy lamino)-5-O-methyl-L-ribonamide.

more info or order :

Alomone Labs product webpage