(R)(+)-Bay K8644 | Alomone Labs B-352 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

(R)(+)-Bay K8644

catalog :

B-352

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs (R)(+)-Bay K8644 inhibits L-type CaVchannels heterologously expressed inXenopusoocytes. - A. Time course of CaV1.2 (co-expressed with ?2?1 and ?1 auxiliary subunits) peak current amplitude elicited by 100 ms voltage ramp from holding potential of -100 mV to +50 mV delivered every 10 seconds. Application of 1 10 and 100 M(R)(+)-Bay K8644(#B-352) inhibits the CaV1.2 current (indicated by the horizontal bar). B. Representative current traces before and during application of 1 10 and 100 M (R)(+)-Bay K8644 as indicated.

product information

CAT :

B-352

SKU :

B-352_1 mg

Product Name :

(R)(+)-Bay K8644

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Storage before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

L-type Ca2+ channels possess binding sites for at least three distinct organic drug types: dihydropyridines (DHP), phenylalkylamines (PAA), and benzothiazepines (BTZ). These binding sites are known to reside in the α1 subunit of the channel.(R)(+)-Bay K8644 is a DHP L-type Ca2+-channel blocker with negative inotropic and vasodilatatory effects in vivo. (R)(+)-Bay K8644 shows opposite effects to (S)(-)-Bay K8644. The action of the of (R)(+)-Bay K8644 was studied in rat tail artery and guinea pig ileal longitudinal smooth muscle using pharmacologic and radioligand binding assays. (R)(+)-Bay K8644 inhibited the responses to KCI-induced depolarization with IC50 values of 16.1-25.6 nM1.

Supplier :

Alomone Labs

Target :

L-type Ca2+ channels

Long Description :

(R)(+)-Bay K8644 (#B-352) is a highly pure, synthetic, and biologically active compound.

Short Description :

A Blocker of L-Type CaV Channels

MW :

356.3

Source :

Synthetic

Molecular formula :

C16H15 F3N2O4.

Effective Concentration :

1-100 µM.

Activity :

(R)(+)-Bay K8644 is an L-type Ca2+-channel blocker1.

Reconstitution and Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeat freeze-thawing may result in loss of activity

Solubility :

100 mM in DMSO. Centrifuge all product preparations before use (10000 x g for 1 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>99% (HPLC)

CAS No :

98791-67-4

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

(4R)-1,4-Dihydro-2,6-dimethyl-5-nitro-4-[2-trifluoromethyl)phenyl]-3-pyridinecarboxylic acid methyl ester.

more info or order :

Alomone Labs product webpage