(S)(-)-Bay K8644 | Alomone Labs B-351 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

(S)(-)-Bay K8644

catalog :

B-351

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Ludwig M, Skorska A, Tolk A, Hopp H, Patejdl R, Li J, et al. Characterization of ion currents of murine CD117(pos) stem cells in vitro and their modulation under AT2 R stimulation. Acta Physiol (Oxf). 2013;208:274-87 pubmed publisher

image

image 1 :

Alomone Labs (S)(-)-Bay K8644 increases L-type CaVchannel currents heterologously expressed inXenopusoocytes. - A. Time course of CaV1.2 (co-expressed with ?2?1 and ?1 auxiliary subunits) peak current amplitude elicited by 100 ms voltage ramp from holding potential of -100 mV to +50 mV delivered every 10 seconds. Application of 0.1 and 1 M(S)(-)-Bay K8644(#B-351) increases the CaV1.2 current (indicated by the horizontal bar). B. Representative current traces before and during application of 0.1 and 1 M (S)(-)-Bay K8644 as indicated.

image 2 :

Alomone Labs LY456066 inhibits mGluR1 mediated Ca2+mobilization in U2OS cells. - Dose-response of normalized inhibition of human mGluR1 mediated L-Glutamate evoked Ca2+mobilization byLY456066(#L-240). IC50was determined at 14.9 nM. hmGluR1-expressing cells were loaded with calcium-sensitive dye incubated with a range of concentrations of LY456066 and stimulated by 15 M L-Glutamate (EC80). Changes in intracellular Ca2+following stimulation were detected as changes in maximum relative fluorescence (RLU) using FLIPRTETRA?.

product information

CAT :

B-351

SKU :

B-351_1 mg

Product Name :

(S)(-)-Bay K8644

Group Type :

Non Antibodies

Product Type :

S. Molecules

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Storage before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

(S)(-)-Bay K8644 is an L-type Ca2+-channel activator with positive inotropic, vasoconstrictive and behavioral effects in vivo. It is an enantiomer of (±)-Bay K8644. (S)(-)-Bay K8644 is a dihydropyridine that can, among other effects, constrict blood vessels and increase the force of contraction of the heart. The drug is thought to increase the entry of Ca2+ into cells by increasing the open-state probability of Ca2+ channels, either by prolonging the duration of individual openings or by increasing the number of openings. It was noted in early experiments with Bay K8644 that the dose-response curve is biphasic1. The action of (S)(-)-Bay K8644 was studied in rat tail artery and guinea pig ileal longitudinal smooth muscle using pharmacologic and radioligand binding assays. It induced concentration-dependent contractions in both smooth muscle preparations with EC50 values of 1.98-11 nM. With increasing concentrations, (S)(-)-Bay K8644 relaxed the tissue with IC50 values of 0.53-2.60 μM2.

Supplier :

Alomone Labs

Target :

L-type Ca2+ channels

Long Description :

(S)(-)-Bay K8644 (#B-351) is a highly pure, synthetic, and biologically active compound.

Short Description :

An Activator of L-Type CaV Channels

MW :

356.3

Source :

Synthetic

Molecular formula :

C16H15 F3N2O4.

Effective Concentration :

50 nM - 5 µM.

Activity :

(S)(-)-Bay K8644 is an L-type Ca2+-channel activator. It displays vasoconstrictive effects in vivo1.

Reconstitution and Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeat freeze-thawing may result in loss of activity

Solubility :

100 mM in DMSO. Centrifuge all product preparations before use (10000 x g for 1 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98% (HPLC)

CAS No :

98625-26-4

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

(4S)-1,4-dihydro-2,6-dimethyl-5-nitro-4-[2-trifluoromethyl)phenyl]-3-pyridinecarboxylic acid methyl ester.

more info or order :

Alomone Labs product webpage