product summary
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company name :
Alomone Labs
product type :
chemical
product name :
(±)-Bay K8644
catalog :
B-350
more info or order :
citations: 10
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image
image 1 :
Alomone Labs ()-Bay K8644 modulates the phosphorylation of ERK1/2 MAPK in Jurkat T cells. - Cells were preincubated with or without EGTA or Nifedipine for 20 min then stimulated with()-Bay K8644(#B-350) or withIonomycinfor 10 min. The cell proteins were blotted and probed with anti-phospho ERK1/2 (upper panel) or with anti-ERK1/2 (lower panel).
product information
CAT :
B-350
SKU :
B-350_10 mg
Product Name :
(±)-Bay K8644
Group Type :
Non Antibodies
Product Type :
S. Molecules
Storage After Reconstitution :
The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
Storage before Reconstitution :
The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.
Shipping and storage :
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Product Page - Scientific background :
(±)-Bay K8644 is an opener of all L-type channels (CaV1 family). In the presence of this agonist, channels tend to open for longer periods causing a large increase in the channel macroscopic response.The net activity of the racemic mix is that of the (-)-enantiomer agonistic activity.(±)-Bay K8644 is a widely used L-type Ca2+ channel activator in various neuronal, muscle, endocrine, thyroid and other cell types.1-3
Supplier :
Alomone Labs
Target :
L-type Ca2+ channels
Long Description :
(±)-Bay K8644 (#B-350) is a highly pure, synthetic, and biologically active compound.
Short Description :
An L-Type Voltage-Gated Ca2+ Channel Activator
MW :
356.3
Source :
Synthetic
Molecular formula :
C16H15F3N2O4.
Effective Concentration :
1-5 μM.
Activity :
(±)-Bay K8644 is a dihydropyridine that acts as an L-type, voltage-gated Ca2+ channel agonist. The (-)-enantiomer has strong Ca2+ channel agonist properties, whereas the (+)-enantiomer has weak Ca2+ channel antagonist activity. The net activity of the racemic mix is L-type channel opening. In the presence of this agonist, channels tend to open for longer periods causing a large increase in the channel microscopic response1-4. (±)-Bay K8644 is also an inhibitor (by increasing the inactivation) of a Shaker KV channel5.
Reconstitution and Solubility :
Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity
Solubility :
Ethanol, methanol or DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions :
Up to four weeks at 4°C or three months at -20°C.
Lead Time :
1-2 Business Days
Country of origin :
Israel/IL
Applications key :
Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.
Purity :
>99% (HPLC)
CAS No :
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Form :
Lyophilized powder.
Comment :
Contact Alomone Labs for technical support and product customization
UNSPSC :
41116134
Bioassay Tested :
Yes
Chemical Name :
1,4-Dihydro-2,6-dimethyl-5-nitro-4-[2-(trifluoromethyl) phenyl]-3-pyridinecarboxylic acid, methyl ester.
more info or order :
company information
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com972 2 531 8002
headquarters: Israel