chemical

BQ788

B-175

B-175_0.5 mg

BQ788

Non Antibodies

S. Molecules

Store the reconstituted solution for the shortest time possible at -20°C. We do not recommend storing the product in working solution for longer than one day. Avoid multiple freeze-thaw cycles.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

BQ788 is a synthetic modified tripeptide that acts as a potent, selective and competitive antagonist of the ET-B (endothelin B) receptor, a receptor that belongs to the G-protein coupled receptor (GPCR) superfamily. BQ788 is a soluble compound that does not bind plasma proteins and is metabolized in a short period of time. It lacks oral bioavailability and requires intra-arterial administration. The compound is used in vitro and in vivo to demonstrate the role of endogenous and exogenous endothelin receptor subtypes in physiological and pathophysiological conditions1,2. It displays IC50 values of 1.2 nM in radiolabeled Girardi heart cells and 1300 nM in human neuroblastoma cell line, respectively1.ET-A and ET-B receptors are widely distributed in vascular tissues where they mediate vasoconstriction and in nonvascular tissues such as the liver, kidney, and brain. ET-A receptors have high affinity for ET-1 and ET-2 peptides and relatively low affinity for ET-3, while ET-B receptors show high affinity for all ET isopeptides1.

Alomone Labs

ET-B receptor

BQ788 (#B-175) is a highly pure, synthetic, and biologically active compound.

A Selective Antagonist of Endothelin B Receptors

641.81

Synthetic

C34H51N5O7.

10-100 nM.

BQ788 is a potent and highly specific ET-B receptor antagonist, exhibiting selectivity for ET-B over ET-A human receptors when inhibiting the binding of [125I]-Endothelin-1 (IC50 of 1.2 nM and 1300 nM, respectively)1.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (50 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity

Soluble in DMSO and ethanol. Centrifuge all products before use (10000 x g 5 min).

Up to three months at -20°C.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>95%

173326-37-9

Lyophilized Powder

Contact Alomone Labs for technical support and product customization

41116134

Yes

(2R)-2-[[(2R)-2-[[(2S)-2-[[(2R,6S)-2,6-dimethylpiperidine-1-carbonyl]amino]-4,4-dimethylpentanoyl]amino]-3-(1-methoxycarbonylindol-3-yl)propanoyl]amino]hexanoic acid.