chemical

Benzamil

B-161

B-161_10 mg

Benzamil

Non Antibodies

S. Molecules

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Benzamil is a potent amiloride analog that acts as an inhibitor of Na+/Ca2+ exchangers and a blocker of ASIC/Deg/ENaC, TRPP3, and TRPA1 channels displaying IC50 of 0.1, 1.1 and 55 µM respectively1-3.Benzamil is commercially available as an inhibitor for acid sensing ion channels (ASICs) belonging to the Deg/ENaC family. The compound is also well known as a diuretic compound that is used for the management of congestive heart disease or hypertension. Intrathecal administration of benzamil demonstrates antinociceptive effects in several pain models in animals and humans through the inhibition of acid sensing ion channels4.

Alomone Labs

Na+/Ca2+ exchangers, ASIC/ENaC, TRPP3, TRPA1 channels

Benzamil (#B-161) is a highly pure, synthetic, and biologically active compound.

An Inhibitor of Na+/Ca2+ Exchangers and a Blocker of ASIC, TRPP3 and TRPA1 Channels

319.75

Benzamil amiloride

Synthetic

C13H14ClN7O.

1-300 µM.

Benzamil is a potent amiloride analogue, an inhibitor of the Na+/Ca2+ exchanger (IC50 = 0.1 µM)1, a blocker of Deg/ENaC channels2, of TRPP3 channels (IC50 = 1.1 µM)3 and of TRPA1 channels (IC50 = 55 µM)4.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. For long-term storage of diluted solutions, we recommend adding 0.1% BSA. Repeat freeze-thawing may result in loss of activity

Up to 100 mM in DMSO. Centrifuge all products before use (10000 x g 5 min).

Up to three months at -20°C.

1-2 Business Days

Israel/IL

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

>99%

161804-20-2

Lyophilized Powder

Contact Alomone Labs for technical support and product customization

41116134

Yes

3,5-diamino-N-(N'-benzylcarbamimidoyl)-6-chloropyrazine-2-carboxamide.