Anti-ZIP14 (SLC39A14) Antibody | Alomone Labs AZT-024 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ZIP14 (SLC39A14) Antibody

catalog :

AZT-024

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4): - 1-2. Anti-ZIP14 (SLC39A14) Antibody (#AZT-024), (1:200). 3-4. Anti-ZIP14 (SLC39A14) Antibody, preincubated with ZIP14 (SLC39A14) Blocking Peptide (BLP-ZT024).

image 2 :

Expression of ZIP14 in mouse striatum. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ZIP14 (SLC39A14) Antibody (#AZT-024), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. ZIP14 immunoreactivity (green) appears in neuron profiles (arrows). B. Pre-incubation of the antibody with ZIP14 (SLC39A14) Blocking Peptide (BLP-ZT024), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AZT-024

SKU :

AZT-024-CF_0.2 ml

Product Name :

Anti-ZIP14 (SLC39A14) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q75N73

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-ZT024

Homology :

Rat - identical, Human - 14 out of 15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, 2nd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KDQEEGVTEKLQNGD, corresponding to amino acid residues 264 - 278 of mouse SLC39A14

Product Page - Scientific background :

Metal ions are essential elements that participate in many different biological processes and therefore their homeostasis is crucial for life. These ions are utilized in oxygen transfer, catalysis, macromolecule stability, gene expression and cell signaling. The homeostasis of metal ions in cells dictated using a specific set of transporters responsible for maintaining their appropriate concentration1. SLC39A14 (ZIP14) is a member of the the Zrt - and - Irt like family (ZIP) a large family of metal ion transporters that can be found in organisms across all kingdoms of life. ZIP14 function as a transporter of zinc, iron, manganese and cadmium across cellular membranes2.According to ZIP14 predicted structure it consists of 8 transmembrane domains (TMDs), extracellular N-terminus and C-terminus and several histidine reach repeats. Structural studies of ZIP14 using SDS-PAGE analysis under nonreducing conditions was show that ZIP14 undergoes trimerization3.Human ZIP14 mRNA was detected in the liver, pancreases, thyroid and in the heart. In addition, mouse mRNA was also detected in the kidney, white adipose tissue (WAT), skeletal muscle and spleen. Mutations in gene encoding ZIP14 leads to manganese toxicity and early-onset dystonia in humans, highlighting ZIP14 important role in manganese homeostasis4.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Zinc Transporter ZIP14, Metal Cation Symporter ZIP14, Zrt- and Irt-like protein 14, Solute Carrier Family 39 Member 14

Short Description :

A Rabbit Polyclonal antibody to ZIP14 (SLC39A14)

Long Description :

Anti-ZIP14 (SLC39A14) Antibody (#AZT-024) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ZIP14 from rat, mouse and human samples.

Negative Control :

BLP-ZT024

Positive Control :

Synonyms :

Zinc Transporter ZIP14, Metal Cation Symporter ZIP14, Zrt- and Irt-like protein 14, Solute Carrier Family 39 Member 14

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC39A14

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200-1:500

more info or order :

Alomone Labs product webpage