Anti-ZnT10 (SLC30A10) Antibody | Alomone Labs AZT-020 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ZnT10 (SLC30A10) Antibody

catalog :

AZT-020

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat small intestine lysate (lanes 1 and 5), mouse liver lysate (lanes 2 and 6), rat liver lysate (lanes 3 and 7) and rat brain lysate (lanes 4 and 8): - 1-4. Anti-ZnT10 (SLC30A10) Antibody (#AZT-020), (1:200). 5-8. Anti-ZnT10 (SLC30A10) Antibody, preincubated with ZnT10 (SLC30A10) Blocking Peptide (BLP-ZT020).

image 2 :

Expression of ZnT10 in rat basal ganglia. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-ZnT10 (SLC30A10) Antibody (#AZT-020), (1:1200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat substantia nigra region, shows ZnT10 immunoreactivity (green) in neuronal profiles (arrows). B. Pre-incubation of the antibody with ZnT10 (SLC30A10) Blocking Peptide (BLP-ZT020), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AZT-020

SKU :

AZT-020-CF_0.2 ml

Product Name :

Anti-ZnT10 (SLC30A10) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q6XR72

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-ZT020

Homology :

Mouse - 13 out of 14 amino acid residues identical; Rat - 12 out of 14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)DASTKIREIFHHAG, corresponding to amino acid residues 361 - 374 of human ZnT10

Product Page - Scientific background :

ZnT10 (also known as SLC30A10) is a member of the Zinc transporter (ZnT) family of proteins that includes 10 members (SLC30A1 to SLC30A10).The SLC30 family reduces the concentration of zinc in the cytoplasm by either exporting it out of the cell or transporting it into intracellular organelles.1ZnT10 was identified as an essential manganese transporter. Manganese is an essential cation for the function of several enzymes, including some crucially important for the metabolism of neurotransmitters and other neuronal metabolic pathways. In fact, mutations in the ZnT10 gene were identified as the underlying cause for hereditary disorders of manganese metabolism leading to hypermanganesemia and accumulation and secondary toxicity in the liver, bone marrow, and the nervous system.2,3ZnT10 also functions as a zinc transporter and can form heterodimers with other ZnT family transporters like ZnT3 and regulate influx of zinc into synaptic vesicles, hence regulating neuronal transmission and plasticity.4

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Zinc Transporter 10, Manganese Transporter SLC30A10, Solute carrier family 30 member 10

Short Description :

A Rabbit Polyclonal Antibody to ZnT10 (SLC30A10)

Long Description :

Anti-ZnT10 (SLC30A10) Antibody (#AZT-020) is a highly specific antibody directed against an epitope of the human ZnT10. The antibody can be used in western blot and immunohistochemistry. It has been designed to recognize ZnT10 from human, mouse and rat samples.

Negative Control :

BLP-ZT020

Positive Control :

Synonyms :

Zinc Transporter 10, Manganese Transporter SLC30A10, Solute carrier family 30 member 10

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC30A10

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:1200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage