Anti-ZnT4 (SLC30A4) Antibody | Alomone Labs AZT-014 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ZnT4 (SLC30A4) Antibody

catalog :

AZT-014

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):

image 2 :

Western blot analysis of rat small intestine lysate:

image 3 :

Western blot analysis of human LNCaP prostate adenocarcinoma cell line lysate (lanes 1 and 3) and human SH-SY5Y neuroblastoma cell line lysates (lanes 2 and 4):

product information

CAT :

AZT-014

SKU :

AZT-014-CF_0.2 ml

Product Name :

Anti-ZnT4 (SLC30A4) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O55174

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-ZT014

Homology :

Mouse - identical; Human - 13 out of 15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DEVSDEGLSRFNKLR, corresponding to amino acid residues 33 - 47 of rat SLC30A4

Product Page - Scientific background :

ZnT4 (also known as Zinc transporter 4 and SLC30A4) is a member of the ZnT family of zinc transporters.Zinc transporters tightly regulate zinc homeostasis. Zinc participates in physiological functions such as DNA synthesis, neurotransmission and brain development. Protein-bound zinc stabilizes and functionalizes proteins, while free zinc acts as a signaling molecule. Zinc defficiency leads to mental lethargy, growth retardation and immune dysfunction, while zinc overload can affect the function of lymphocytes and cause copper deficiency. 1Zinc transporters are classified into two families: The ZnT (SLC30) family and the ZIP (SLC39) family. Most ZnT proteins form homodimers and have six transmembrane domains, with a long His-rich loop between these domains, which may function as a sensor of cytosolic zinc levels. The C- and N- termini of ZnTs are located inside the cytoplasm. ZnT proteins are efflux transporters that reduce cytosolic zinc by transporting it out of the cell or into intracellular compartments. In contrast, ZIP proteins are influx transporters that form homodimers or hetrodimers. They have eight transmembrane domains, and their C- and N- termini are both located outside the plasma membrane or in the lumen of a subcellular compartment.2ZnT4 is widely expressed in humans, with notable enrichment in the brain as well as several other organs, including the thyroid, lung, testis, heart, skin and pancreas. ZnT4 is expressed in endosomes/lysosomes, the Golgi apparatus and cytoplasmic vesicles, where it is involved in vesicular secretory functions.3There is increasing evidence that zinc transporters are connected with the formation of senile plaques in Alzheimer's Disease (AD). Abundant expression and altered distribution of ZnT4 was present in the entire body of the senile plaques of APP/PS1 mice and human AD brains.1 Also, it has been shown that ZnT4 mutant mice develop balance and spatial memory behavioral abnormalities.4Expression of ZnT4 in the mammary gland has been reported in mouse, where it has been shown to cause the lethal milk syndrome (LMS). Mice with LMS are not zinc deficient but females cannot produce milk containing sufficient zinc for the pups to survive. 4

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Zinc transporter 4, Solute carrier family 30 member 4

Short Description :

A Rabbit Polyclonal antibody to GPRC5B receptor

Long Description :

Anti-ZnT4 (SLC30A4) Antibody (#AZT-014) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ZnT4 from rat, mouse and human samples.

Negative Control :

BLP-ZT014

Positive Control :

Synonyms :

Zinc transporter 4, Solute carrier family 30 member 4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC30A4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:400-1:500

more info or order :

Alomone Labs product webpage