Anti-ZnT3 (SLC30A3) Antibody | Alomone Labs AZT-013 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ZnT3 (SLC30A3) Antibody

catalog :

AZT-013

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Diethorn E, Gould E. Postnatal development of hippocampal CA2 structure and function during the emergence of social recognition of peers. Hippocampus. 2022;: pubmed publisher
Licht T, Kreisel T, Biala Y, Mohan S, Yaari Y, Anisimov A, et al. Age-Dependent Remarkable Regenerative Potential of the Dentate Gyrus Provided by Intrinsic Stem Cells. J Neurosci. 2020;40:974-995 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-ZnT3 (SLC30A3) Antibody (#AZT-013), (1:500).3,4. Anti-ZnT3 (SLC30A3) Antibody, preincubated with ZnT3/SLC30A3 Blocking Peptide (#BLP-ZT013).

image 2 :

Expression of Zinc transporter 3 in rat hippocampus - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-ZnT3 (SLC30A3) Antibody (#AZT-013), (1:600). A. ZnT3 (green) is expressed in the mossy fiber (MF) terminal field of the CA3 region rat hippocampal region. B. Synaptophysin staining (red) is detected in both the MF terminal field and the CA3 region. C. Merge of the two images reveals extensive co-localization (arrows) in the MF region. Cell nuclei are stained with DAPI (blue).

image 3 :

Western blot analysis of human SH-SY5Y neuroblastoma cell lysate: - 1.Anti-ZnT3 (SLC30A3) Antibody(#AZT-013) (1:200).2. Anti-ZnT3 (SLC30A3) Antibody preincubated with the negative control antigen.

product information

CAT :

AZT-013

SKU :

AZT-013-CF_0.2 ml

Product Name :

Anti-ZnT3 (SLC30A3) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q6QIX3

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-ZT013

Homology :

Mouse - 12/14 amino acid residues identical; human - 11/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RGAEYAPLEEGHGH, corresponding to amino acid residues 210-223 of rat ZnT3

Product Page - Scientific background :

ZnT3 is a member of the SLC30 family of zinc transporters which promotes the influx of zinc ions into synaptic vesicles of glutamatergic neurons from the cytoplasm, intracellular organelles, or to the outside of the cell. It is responsible for maintaining high intravesicular zinc content.ZnT3 is encoded by the Slc30a3 gene and is selectively located on the vesicles of zinc-secreting neurons. ZnT3 is also present in regions of the brain such as the hippocampus and neocortex1. Ionic zinc is found ubiquitously throughout synapses of the mammalian central nervous system, where it plays fundamental roles in synaptic function and plasticity2.ZnT3 structure is predicted to contain six transmembrane spanning domains that form a pore lined with a histidine-rich loop3.Knockout of ZnT3 leads to the absence of histochemically reactive zinc in the terminals of zincergic neurons. A loss of ZnT3 might lead to disturbances in brain functions such as memory function, emotions, behavior, motivation, and in the processing of sensory information3,4. Studies have shown that genetic absence of ZnT3 may be involved in the synaptic and memory deficits of Alzheimer's disease1. Another study showed that a knockout mouse has a tendency for seizure5.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Zinc transporter 3

Short Description :

A Rabbit Polyclonal Antibody to Zinc Transporter 3

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat zinc transporter 3. Anti-ZnT3 (SLC30A3) Antibody (#AZT-013) can be used in western blot and immunohistochemistry applications. It has been designed to recognize ZnT3 from mouse, rat and human samples.

Negative Control :

BLP-ZT013

Positive Control :

Synonyms :

Zinc transporter 3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC30A3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:600

Application Dilutions: Western blot wb :

1:200-1:500

more info or order :

Alomone Labs product webpage