Anti-ZnT1 (SLC30A1) Antibody | Alomone Labs AZT-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ZnT1 (SLC30A1) Antibody

catalog :

AZT-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Perna F, Berman S, Soni R, Mansilla Soto J, Eyquem J, Hamieh M, et al. Integrating Proteomics and Transcriptomics for Systematic Combinatorial Chimeric Antigen Receptor Therapy of AML. Cancer Cell. 2017;32:506-519.e5 pubmed publisher

image

image 1 :

Western blot analysis of mouse lung lysate (lanes 1 and 4), rat liver lysate (lanes 2 and 5) and rat brain membranes (lanes 3 and 6): - 1-3. Anti-ZnT1 (SLC30A1) Antibody (#AZT-011), (1:400).4-6. Anti-ZnT1 (SLC30A1) Antibody, preincubated with ZnT1/SLC30A1 Blocking Peptide (#BLP-ZT011).

image 2 :

Expression of Zinc transporter 1 in rat olfactory bulb - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-ZnT1 (SLC30A1) Antibody (#AZT-011), (1:300), followed by goat anti-rabbit-AlexaFluor-488 antibody. ZnT1 staining (green) appears in glomeruli (G) and is particularly intense in the outer border (arrows). Cell nuclei are labeled with DAPI (blue).

product information

CAT :

AZT-011

SKU :

AZT-011-CF_0.2 ml

Product Name :

Anti-ZnT1 (SLC30A1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q62720

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-ZT011

Homology :

Mouse, human - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)ENLEKKPRRTKAE, corresponding to amino acid residues 473-485 of rat ZnT1

Product Page - Scientific background :

ZnT1 is a member of the SLC30 family of zinc transporters which promotes the influx of zinc ions into synaptic vesicles of glutamatergic neurons from the cytoplasm, intracellular organelles, and from the extracellular milieu.Zinc plays an essential role in many physiological and pathological conditions such as synaptic plasticity modulation, memory formation and nociception by regulating transmitter receptors and signal transduction pathways. Therefore zinc homeostasis is tightly regulated at the cellular and the organismal levels1,2.ZnT1 in mammals is localized primarily on the plasma membrane. Its role is to mediate zinc export to reduce intracellular zinc levels1. ZnT1 structure contains a PDZ I motif at the C-terminus and six transmembrane-spanning domains. There is also a (His-Gly)8 in the large intracellular loop between membrane-spanning regions 4 and 52,4.ZnT1 expression is ubiquitous and is detected in the intestine, kidney and liver. In the intestine the expression is regional, being much greater in the duodenum and jejunum and in villus versus crypt cells.The mechanism of ZnT1 regulation is under control of the zinc-responsive transcription factor MTF-11. Research shows that homozygous knockout of the ZnT1 gene is lethal to the foetus3.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Zinc transporter 1, ZRC 1

Short Description :

A Rabbit Polyclonal Antibody to Zinc Transporter 1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Zinc transporter 1. Anti-ZnT1 (SLC30A1) Antibody (#AZT-011) can be used in western blot and immunohistochemistry applications. It has been designed to recognize ZnT1 from mouse, rat, and human samples.

Negative Control :

BLP-ZT011

Positive Control :

Synonyms :

Zinc transporter 1, ZRC 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC30A1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage