Anti-ZIP10/SLC39A10 (extracellular) Antibody | Alomone Labs AZT-010 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ZIP10/SLC39A10 (extracellular) Antibody

catalog :

AZT-010

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):

image 2 :

Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 4), human THP-1 monocytic leukemia cell line lysate (lanes 2 and 5) and human K562 chronic myelogenous leukemia cell line lysate (lanes 3 and 6):

image 3 :

Expression of ZIP10 in mouse hippocampus

product information

CAT :

AZT-010

SKU :

AZT-010-CF_0.2 ml

Product Name :

Anti-ZIP10/SLC39A10 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q6P5F6

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-ZT010

Homology :

Rat - identical Human - 14 out of 15 amino acid identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)EQYEHNRVHKLDRVH, corresponding to amino acid residues 259 - 273 of mouse SLC39A10

Product Page - Scientific background :

ZIP10, also known as Solute Carrier Family 39 Member 10 (SLC39A10), is a zinc transporter protein that belongs to the ZIP (Zrt-, Irt-like Protein) family.1-3The ZIP family includes 14 members (ZIP1 to ZIP14), that play a critical role in the transport of metal ions, primarily zinc, iron, and manganese, across cell membranes. ZIP family members are primarily involved in the uptake of zinc and iron into the cytoplasm from the extracellular space or from intracellular organelles. Their function mostly increases the cytoplasmic concentration of these metals by transporting them across cellular membranes.1-3ZIP10 is expressed at the cell membrane and serves as a zinc importer into cells. ZIP10 expression is also modulated by the available zinc concentration: upregulated in the presence of low zinc concentrations and downregulated when zinc is present in excess. 2,3ZIP10 plays a central role in immune function regulation in both the adaptive and innate immune system. ZIP10 regulates B-cell and T-cell development and function as well as having a role in the modulation of the inflammatory response in macrophages. 4,5ZIP10 is expressed in several organs like kidney and the central nervous system, but its exact physiological function is still not clear.Dysregulated zinc homeostasis is linked to the development and progression of several cancers and ZIP10, in particular, has garnered attention for its involvement in cancer biology in particular through its heteromer formation with ZIP6, another ZIP family member.2,6-8ZIP10 has been shown to be involved in the regulation of cell proliferation, cell migration and invasion and the regulation of apoptosis, in several cancer types including pancreatic, breast and prostate, suggesting that the inhibition of ZIP10 function could represent a therapeutic target in these and other tumors.6-8

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Zinc transporter ZIP10, Zrt- and Irt-like protein 10, Solute Carrier Family 39 Member 10

Short Description :

A Rabbit Polyclonal Antibody to ZIP10/SLC39A10 (extracellular)

Long Description :

Anti-ZIP10/SLC39A10 (extracellular) Antibody (#AZT-010) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize ZIP10 from mouse, rat and human samples.

Negative Control :

BLP-ZT010

Positive Control :

Synonyms :

Zinc transporter ZIP10, Zrt- and Irt-like protein 10, Solute Carrier Family 39 Member 10

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC39A10

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage