antibody

Anti-ZIP10/SLC39A10 (extracellular)-FITC Antibody

AZT-010-F

polyclonal

domestic rabbit

nonconjugated

human, mouse, rat

AZT-010-F_15 mcl

Anti-ZIP10/SLC39A10 (extracellular)-FITC Antibody

Antibodies

Antibodies

Polyclonal

Q6P5F6

FC LCI

Human Rat Mouse

Rabbit

BLP-ZT010

Rat – identical.  Human – 14 out of 15 amino acid identical.

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1% BSA, 0.05% NaN3

NA

Fluorescein isothiocyanate (FITC).

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

(C)EQYEHNRVHKLDRVH, corresponding to amino acid residues 259 - 273 of mouse SLC39A10

ZIP10, also known as Solute Carrier Family 39 Member 10 (SLC39A10), is a zinc transporter protein that belongs to the ZIP (Zrt-, Irt-like Protein) family.1-3 The ZIP family includes 14 members (ZIP1 to ZIP14), that play a critical role in the transport of metal ions, primarily zinc, iron, and manganese, across cell membranes. ZIP family members are primarily involved in the uptake of zinc and iron into the cytoplasm from the extracellular space or from intracellular organelles. Their function mostly increases the cytoplasmic concentration of these metals by transporting them across cellular membranes.1-3 ZIP10 is expressed at the cell membrane and serves as a zinc importer into cells. ZIP10 expression is also modulated by the available zinc concentration: upregulated in the presence of low zinc concentrations and downregulated when zinc is present in excess. 2,3 ZIP10 plays a central role in immune function regulation in both the adaptive and innate immune system. ZIP10 regulates B-cell and T-cell development and function as well as having a role in the modulation of the inflammatory response in macrophages. 4,5 ZIP10 is expressed in several organs like kidney and the central nervous system, but its exact physiological function is still not clear. Dysregulated zinc homeostasis is linked to the development and progression of several cancers and ZIP10, in particular, has garnered attention for its involvement in cancer biology in particular through its heteromer formation with ZIP6, another ZIP family member.2,6-8 ZIP10 has been shown to be involved in the regulation of cell proliferation, cell migration and invasion and the regulation of apoptosis, in several cancer types including pancreatic, breast and prostate, suggesting that the inhibition of ZIP10 function could represent a therapeutic target in these and other tumors.6-8

FC LCI

Alomone Labs

Zinc transporter ZIP10, Zrt- and Irt-like protein 10, Solute Carrier Family 39 Member 10

A Rabbit Polyclonal Antibody to ZIP10/SLC39A10 conjugated to fluorescein isothiocyanate (FITC) fluorophore

Anti-ZIP10/SLC39A10 (extracellular) Antibody (#AZT-010) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize ZIP10 from mouse, rat and human samples. Anti-ZIP10/SLC39A10 (extracellular)-FITC Antibody (#AZT-010-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

RIC-001-F

Zinc transporter ZIP10, Zrt- and Irt-like protein 10, Solute Carrier Family 39 Member 10

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

SLC39A10

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

Affinity purified on immobilized antigen.

Rabbit IgG Isotype Control-FITC

41116161

Western blot analysis (unlabeled antibody, #AZT-010), and direct flow cytometry (labeled antibody).