Anti-ZIP6/SLC39A6 (extracellular) Antibody | Alomone Labs AZT-006 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ZIP6/SLC39A6 (extracellular) Antibody

catalog :

AZT-006

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: - 1,2. Anti-ZIP6/SLC39A6 (extracellular) Antibody (#AZT-006), (1:200).3,4. Anti-ZIP6/SLC39A6 (extracellular) Antibody, preincubated with ZIP6/SLC39A6 (extracellular) Blocking Peptide (#BLP-ZT006).

image 2 :

Expression of ZIP6 in rat hypothalamus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-ZIP6/SLC39A6 (extracellular) Antibody (#AZT-006), (1:200), followed by goat anti-rabbit-AlexaFluor-488. ZIP6 immunoreactivity (green) appears in the median eminence (ME, arrows). Cell nuclei are stained with DAPI (blue). 3V = Third ventricle.

product information

CAT :

AZT-006

SKU :

AZT-006-CF_0.2 ml

Product Name :

Anti-ZIP6/SLC39A6 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8C145

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-ZT006

Homology :

Rat, human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)ESVSEPRKSFMYSR, corresponding to amino acid residues 251-264 of mouse ZIP6

Product Page - Scientific background :

ZIP6 (SLC39A6) is a zinc (Zn) transporter located on the cell membrane.Zn is an essential heavy metal, part of numerous biological processes as an enzymatic co-factor, and also as an intracellular signaling mediator that recruits extracellular stimuli and determines the final outputs of various physiological pathways. Intracellular and extracellular Zn levels regulate many cellular processes such as autophagy, cell growth and death, and cell migration1,2.Movement of Zn in and out of cells, across membranes, is regulated by zinc transporters. Zn transporter protein 6 (ZIP6) is a member of the ZIP family of Zinc transporters and of the LIV-1 sub-family. ZIP transporters contain 8 transmembrane domains with a cytoplasmic region between TM3 and TM4. LIV-1 subfamily members contain the HEXXH motif within TM5. They also exhibit diverse lengths of the N-terminal extracellular domain3.The gene encoding this transporter has been shown to take part in estrogen receptor-positive breast cancer and serves as a reliable marker of this disease. Overexpression of ZIP6 protein is also observed in prostate and kidney tumor cells4.ZIP6 is also strongly upregulated during T-cell activation5. ZIP6 heteromerizes with ZIP10 for oocyte-to-egg transition and epithelial to mesenchymal transition in fetus development6,7.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Zinc transporter ZIP6, Zrt- and Irt-like protein 6, Estrogen-regulated protein LIV-1, LIV1, Solute carrier family 39 member 6

Short Description :

A Rabbit Polyclonal Antibody to Zinc Transporter ZIP6

Long Description :

Anti-ZIP6/SLC39A6 (extracellular) Antibody (#AZT-006) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize ZIP6 from rat, mouse, and human samples.

Negative Control :

BLP-ZT006

Positive Control :

Synonyms :

Zinc transporter ZIP6, Zrt- and Irt-like protein 6, Estrogen-regulated protein LIV-1, LIV1, Solute carrier family 39 member 6

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC39A6

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage