Anti-ZIP6/SLC39A6 (extracellular)-FITC Antibody | Alomone Labs AZT-006-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ZIP6/SLC39A6 (extracellular)-FITC Antibody

catalog :

AZT-006-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of ZIP6 by direct flow cytometry in live intact mouse TK-1 T-cell lymphoma cells: Cell surface detection of ZIP6 by direct flow cytometry in live intact mouse TK-1 T-cell lymphoma cells: Black line: Cells. Red line: Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F). Green line: Cells + Anti-ZIP6/SLC39A6 (extracellular)-FITC Antibody (#AZT-006-F), (2.5µg).

product information

CAT :

AZT-006-F

SKU :

AZT-006-F_15 mcl

Product Name :

Anti-ZIP6/SLC39A6 (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8C145

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-ZT006

Homology :

Rat, human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)ESVSEPRKSFMYSR, corresponding to amino acid residues 251-264 of mouse ZIP6

Product Page - Scientific background :

ZIP6 (SLC39A6) is a zinc (Zn) transporter located on the cell membrane.Zn is an essential heavy metal, part of numerous biological processes as an enzymatic co-factor, and also as an intracellular signaling mediator that recruits extracellular stimuli and determines the final outputs of various physiological pathways. Intracellular and extracellular Zn levels regulate many cellular processes such as autophagy, cell growth and death, and cell migration1,2.Movement of Zn in and out of cells, across membranes, is regulated by zinc transporters. Zn transporter protein 6 (ZIP6) is a member of the ZIP family of Zinc transporters and of the LIV-1 sub-family. ZIP transporters contain 8 transmembrane domains with a cytoplasmic region between TM3 and TM4. LIV-1 subfamily members contain the HEXXH motif within TM5. They also exhibit diverse lengths of the N-terminal extracellular domain3.The gene encoding this transporter has been shown to take part in estrogen receptor-positive breast cancer and serves as a reliable marker of this disease. Overexpression of ZIP6 protein is also observed in prostate and kidney tumor cells4.ZIP6 is also strongly upregulated during T-cell activation5. ZIP6 heteromerizes with ZIP10 for oocyte-to-egg transition and epithelial to mesenchymal transition in fetus development6,7.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Zinc transporter ZIP6, Zrt- and Irt-like protein 6, Estrogen-regulated protein LIV-1, LIV1, Solute carrier family 39 member 6

Short Description :

A Rabbit Polyclonal Antibody to ZIP6 (extracellular) conjugated to the fluorescent dye FITC

Long Description :

Anti-ZIP6/SLC39A6 (extracellular) Antibody (#AZT-006) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize ZIP6 from rat, mouse and human samples. Anti-ZIP6/SLC39A6 (extracellular)-FITC Antibody (#AZT-006-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize ZIP6 from rat, mouse and human samples.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Zinc transporter ZIP6, Zrt- and Irt-like protein 6, Estrogen-regulated protein LIV-1, LIV1, Solute carrier family 39 member 6

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC39A6

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AZT-006), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage