Anti-ZIP3 (SLC39A3) Antibody | Alomone Labs AZT-003 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ZIP3 (SLC39A3) Antibody

catalog :

AZT-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse lung (lanes 1 and 4), rat brain (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates: - 1-3. Anti-ZIP3 (SLC39A3) Antibody (#AZT-003), (1:400).4-6. Anti-ZIP3 (SLC39A3) Antibody, preincubated with ZIP3/SLC39A3 Blocking Peptide (#BLP-ZT003).

image 2 :

Expression of Zinc transporter ZIP3 in rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-ZIP3 (SLC39A3) Antibody (#AZT-003), (1:400), followed by goat-anti-rabbit-AlexaFluor-488 antibody. ZIP3 staining (green) appears in CA1 neurons in the pyramidal layer (arrow). Nuclei are labeled with DAPI (blue).

product information

CAT :

AZT-003

SKU :

AZT-003-CF_0.2 ml

Product Name :

Anti-ZIP3 (SLC39A3) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q5U1X7

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-ZT003

Homology :

Mouse, human - 11/12 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EADFEKAHRSKK, corresponding to amino acid residues 31-42 of rat ZIP3

Product Page - Scientific background :

Zinc uptake transporter, ZIP3, belongs to the SLC39 transporter family. ZIP transporters are responsible for zinc transport into the cytoplasm across cellular membranes, either influx from the extracellular space or efflux from intracellular organelles1. ZIP3 structure includes eight transmembrane (TM) domains with extracellular N- and C-termini1.Mammalian ZIP3 is encoded by the SLC39A3 gene and its expression is detected in tissues with unique requirements for Zn2+. High levels of ZIP3 are noticed in specialized hormonally-responsive secretory tissues, such as the pancreas, prostate and mammary gland2.Regulation and maintenance of proper concentration and distribution of cellular zinc nutrient by ZIP transporters are essential to the function, metabolism, growth, proliferation and survival of cells. Zinc deficiency can cause growth retardation, cognitive impairment, metabolic disorders, infertility, dysfunction of the immune system, including abnormalities in T-cells, natural killer cells and monocytes, and reduction in antibody formation1. However, excess zinc can be toxic, therefore, zinc transport plays a crucial role in regulating cellular zinc homeostasis and its levels are tightly regulated by the activity of zinc transporters.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Zinc transporter ZIP3, Zrt- and Irt-like protein 3

Short Description :

A Rabbit Polyclonal Antibody to Zinc Transporter ZIP3

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Zinc transporter ZIP3. Anti-ZIP3 (SLC39A3) Antibody (#AZT-003) can be used in western blot and immunohistochemistry applications. It has been designed to recognize ZIP3 from mouse, rat and human samples.

Negative Control :

BLP-ZT003

Positive Control :

Synonyms :

Zinc transporter ZIP3, Zrt- and Irt-like protein 3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC39A3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage