Anti-ZIP1 (SLC39A1) (extracellular) Antibody | Alomone Labs AZT-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ZIP1 (SLC39A1) (extracellular) Antibody

catalog :

AZT-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-ZIP1 (SLC39A1) (extracellular) Antibody (#AZT-001), (1:200).3,4. Anti-ZIP1 (SLC39A1) (extracellular) Antibody, preincubated with ZIP1/SLC39A1 (extracellular) Blocking Peptide (#BLP-ZT001).

image 2 :

Expression of ZIP1 in rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-ZIP1 (SLC39A1) (extracellular) Antibody (#AZT-001), (1:400), followed by anti-rabbit-Alexa-488 antibody. ZIP1 is expressed (green) in the mossy fiber (MF) terminal field (arrows) of the CA3 hippocampal region. Nuclei are labeled with DAPI (blue).

image 3 :

Western blot analysis of human U-87 MG glioblastoma cell lysate (lanes 1 and 3) and human MDA-231 breast adenocarcinoma cell lysate (lanes 2 and 4): - 12.Anti-ZIP1 (SLC39A1) (extracellular) Antibody(#AZT-001) (1:200).34. Anti-ZIP1 (SLC39A1) (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

AZT-001

SKU :

AZT-001-CF_0.2 ml

Product Name :

Anti-ZIP1 (SLC39A1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9NY26

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-ZT001

Homology :

Mouse, rat - 15/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)GEPELLVWRPEAVASE, corresponding to amino acid residues 5-20 of human ZIP1

Product Page - Scientific background :

Zinc uptake transporter, ZIP1, belongs to the SLC39 transporter family. This transport has the ability to increase zinc concentration when cellular zinc level is low, by active zinc transport into the cytoplasm from extracellular space of the cell or the intracellular storage compartment. Thus, zinc transport plays a crucial role in regulating cellular zinc homeostasis1-3.Regulation and maintenance of proper concentration and distribution of cellular zinc are essential to the function, metabolism, growth, proliferation and survival of cells. Zinc deficiency can cause defects in the immune system, including abnormalities in T-cells, natural killer cells and monocytes, and reduction in antibody formation1,2.In the human genome, 20 ZIP transporters have been identified. ZIP1 is encoded by the SLC39A1 gene3.ZIP proteins are highly conserved. Members have eight predicted transmembrane (TM) domains with extracellular N- and C-termini. There is a long loop region harboring a histidine-rich domain between TM3 and TM4. Zinc uptake by ZIP1 is energy independent2.ZIP1 is ubiquitously expressed in human tissues including blood, brain, gill, heart, intestine, liver, muscle, skin, spleen and kidney3.Accumulation of zinc in prostate cells by ZIP1 inhibits their growth and proliferation. Therefore, ZIP1 is associated as a tumor suppressor gene in prostate cancer2.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Zinc transporter ZIP1, Zinc-iron-regulated transporter-like, Zrt- and Irt-like protein 1, ZIRTL

Short Description :

A Rabbit Polyclonal Antibody to Zinc Transporter ZIP1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human Zinc transporter ZIP1. Anti-ZIP1 (SLC39A1) (extracellular) Antibody (#AZT-001) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize ZIP1 from mouse, rat and human samples.

Negative Control :

BLP-ZT001

Positive Control :

Synonyms :

Zinc transporter ZIP1, Zinc-iron-regulated transporter-like, Zrt- and Irt-like protein 1, ZIRTL

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC39A1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage