Anti-Human ZACN (extracellular) Antibody | Alomone Labs AZC-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human ZACN (extracellular) Antibody

catalog :

AZC-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of PANC-1 (lanes 1 and 3) and Jurkat (lanes 2 and 4) cell lysates: - 1,2. Anti-Human ZACN (extracellular) Antibody (#AZC-001), (1:200).3,4. Anti-Human ZACN (extracellular) Antibody, preincubated with Human ZACN (extracellular) Blocking Peptide (#BLP-ZC001).

image 2 :

Cell surface detection of ZAC in Jurkat living cells: - ___Unstained cells.___Cells +Anti-Human ZACN (extracellular) Antibody(#AZC-001) (5-10g/1x106cells).

product information

CAT :

AZC-001

SKU :

AZC-001-CF_0.2 ml

Product Name :

Anti-Human ZACN (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q401N2

Applications :

IC IF IFC LCI WB

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-ZC001

Homology :

Human

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CNFELLHFPRDHSN, corresponding to amino acid residues 157-170 of human ZACN

Product Page - Scientific background :

Over forty homologous proteins belong to the superfamily of ligand-gated ion channels. These members are divided into subfamilies, for example: acetylcholine, serotonin and glycine receptors.A new member has been added to this superfamily - ZAC (Zinc-activated channel) which is expressed in humans and dogs but not in rodents1. Like all other ligand-gated ion channels, ZAC has four transmembrane domains and extracellular N- and C-termini. As its name states, the channel is activated by high concentrations of zinc.ZAC mRNA was found to be expressed in the placenta, spinal cord, stomach, and fetal brain1. In another study, ZAC was identified using a computer-assisted program and was detected in similar areas (including pancreas and kidney), but also displayed minor discrepancies2.Being that the channel is expressed in human and dog, deciphering its cellular/physiological functions will be challenging.

Applications may also work in :

IC IF IFC LCI WB

Supplier :

Alomone Labs

Target :

Zinc-activated ligand-gated ion channel, Ligand-gated ion channel zinc-activated 1, Ligand-gated ion-channel receptor L2, ZAC, LGICZ1

Short Description :

A Rabbit Polyclonal Antibody to Zinc-Activated Ion Channel

Long Description :

Anti-Human ZACN (extracellular) Antibody (#AZC-001) is a highly specific antibody directed against an epitope of the human Zinc-activated ligand-gated ion channel. The antibody can be used in western blot, immunocytochemistry, and indirect flow cytometry applications. The antibody recognizes an extracellular epitope, and is thus ideal for detecting ZAC in living cells. It has been designed to recognize ZAC from human samples only.

Negative Control :

BLP-ZC001

Positive Control :

Synonyms :

Zinc-activated ligand-gated ion channel, Ligand-gated ion channel zinc-activated 1, Ligand-gated ion-channel receptor L2, ZAC, LGICZ1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ZACN

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage