Anti-Oxytocin Receptor Antibody | Alomone Labs AVR-013 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Oxytocin Receptor Antibody

catalog :

AVR-013

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Alomone Labs product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; rat; 1:100; loading ...; fig 12a	Warfvinge K, KRAUSE D, Edvinsson L. The distribution of oxytocin and the oxytocin receptor in rat brain: relation to regions active in migraine. J Headache Pain. 2020;21:10 pubmed publisher
	Salehi M, Neumann I, Jurek B, Pandamooz S. Co-Stimulation of Oxytocin and Arginine-Vasopressin Receptors Affect Hypothalamic Neurospheroid Size. Int J Mol Sci. 2021;22: pubmed publisher
	Xiao L, Priest M, Nasenbeny J, Lu T, Kozorovitskiy Y. Biased Oxytocinergic Modulation of Midbrain Dopamine Systems. Neuron. 2017;95:368-384.e5 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membrane (1:200), rat testis and mouse testis lysates (1:400): - 1-3. Anti-Oxytocin Receptor Antibody (#AVR-013).4-6. Anti-Oxytocin Receptor Antibody preincubated with Oxytocin Receptor Blocking Peptide (#BLP-VR013).

image 2 :

Expression of Oxytocyn Receptor in rat midbrain. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Oxytocin Receptor Antibody (#AVR-013), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Oxytocyn Receptor immunoreactivity (green) appears in neuronal profiles (arrows) in the substantia nigra pars compacta (SNC). B. Pre-incubation of the antibody with Oxytocin Receptor Blocking Peptide (#BLP-VR013), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AVR-013

SKU :

AVR-013-CF_0.2 ml

Product Name :

Anti-Oxytocin Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P70536

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-VR013

Homology :

Mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CSARYLKGSRPGE, corresponding to amino acid residues 346-358 of rat Oxtr

Product Page - Scientific background :

Oxytocin receptor belongs to the class 1 family of G-protein coupled receptors (GPCR) and its role is to regulate reproductive physiology, social and emotional behaviors. Like other members belonging to the GPCR superfamily, it has seven transmembrane domains, extracellular N-terminus and intracellular C-terminal tail1,2.The Oxytocin receptor ligand oxytocin, is a peptide that functions both as a hormone and as a neurotransmitter, and plays an important role in social behavior, estrous cycle, induction of labor and milk ejection, anxiety-related behaviors, food intake, stress-related responses, and pain control. Activation of the receptor leads to the stimulation of phospholipase C by interacting with Gαq/11 in response to ligand binding1.Oxytocin receptor is widely abundant in several brain regions including the basal ganglia, the limbic system, the thalamus, the hypothalamus, the brainstem, and the spinal cord. It is also detected in the uterus, mammary gland, ovary, testis, prostate and kidney.Several studies have suggested that administration of oxytocin in humans has the ability to increase generosity, trust and eye gaze. Oxytocin levels in the plasma relates to parent-child bonding behaviors, feelings of romantic love, trust and empathy. Similarly, Oxytocin receptor knockout mice shows several abnormalities in social and emotional behavior such as increased aggression and deficits in nurturing, positive affection, attachment, sensitivity and social memory2,3.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

OXTR, OT-R, OTR1

Short Description :

A Rabbit Polyclonal Antibody to Oxytocin Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Oxytocin receptor. Anti-Oxytocin Receptor Antibody (#AVR-013) can be used in western blot analysis. It has been designed to recognize the oxytocin receptor from rat and mouse samples. The antibody is not recommended for use with human samples.

Negative Control :

BLP-VR013

Positive Control :

Synonyms :

OXTR, OT-R, OTR1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

OXTR

AB Specifictiy :

Not recommended for use with human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage