Anti-Vasopressin V2 Receptor (AVPR2) Antibody | Alomone Labs AVR-012 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Vasopressin V2 Receptor (AVPR2) Antibody

catalog :

AVR-012

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Masuda T, Muto S, Fukuda K, Watanabe M, Ohara K, Koepsell H, et al. Osmotic diuresis by SGLT2 inhibition stimulates vasopressin-induced water reabsorption to maintain body fluid volume. Physiol Rep. 2020;8:e14360 pubmed publisher
Maruyama H, Taguchi A, Nishikawa Y, Guili C, Mikame M, Nameta M, et al. Medullary thick ascending limb impairment in the GlatmTg(CAG-A4GALT) Fabry model mice. FASEB J. 2018;32:4544-4559 pubmed publisher
Mamenko M, Dhande I, Tomilin V, Zaika O, Boukelmoune N, Zhu Y, et al. Defective Store-Operated Calcium Entry Causes Partial Nephrogenic Diabetes Insipidus. J Am Soc Nephrol. 2016;27:2035-48 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) kidney lysates: - 1,2. Anti-Vasopressin V2 Receptor (AVPR2) Antibody (#AVR-012), (1:200).3,4. Anti-Vasopressin V2 Receptor (AVPR2) Antibody, preincubated with Vasopressin V2 Receptor/AVPR2 Blocking Peptide (#BLP-VR012).

image 2 :

Peptide RHTTHSLGPQDESC corresponding to amino acid residues 345-358 of rat AVPR2(AccessionQ00788). Intracellular C-terminus.

image 3 :

Cell surface detection of p75NTR inlive intact rat pheochromocytoma (PC12) cells: - ___ Unstained cells.___ Cells + Anti-p75NGF Receptor (extracellular)-ATTO-488 Antibody(#ANT-007-AG) (1:20).

product information

CAT :

AVR-012

SKU :

AVR-012-CF_0.2 ml

Product Name :

Anti-Vasopressin V2 Receptor (AVPR2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q00788

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-VR012

Homology :

Mouse - identical; human - 9/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

RHTTHSLGPQDESC, corresponding to amino acid residues 345-358 of rat AVPR2

Product Page - Scientific background :

Vasopressin V2 receptor (V2R), or arginine vasopressin receptor 2 (AVPR2), is a G-protein-coupled receptor (GPCR), coupled to the stimulatory heterotrimeric GTP-binding protein, GS, that elevates intracellular cAMP through activation of adenylate cyclase1-3. Like all members, it has seven transmembrane domains, an extracellular N-terminus and intracellular C-terminal tail1.AVPR2 is expressed primarily in renal collecting ducts and loop of Henle of the kidney, but also in vascular smooth muscle, where it induces vasodilation. Its ligand is the anti-diuretic hormone (ADH), aka vasopressin; a hormone that originates from the posterior pituitary gland that has key roles in water homeostasis and in hypotension5.Vasopressin triggers a variety of receptors - V1R, V2R, V3R, oxytocin and purinergic receptors, which regulate the physiological water-balance. High levels of inflammatory factors in the blood, such as the case in sepsis, are known to down-regulate the vasopressin receptors3.Mutations in V2R that prevent the receptor from reaching and anchoring to the cell surface are responsible are detected in diabetes insipidus and heart failure4,6,7 just to name a few. Interestingly, it was found that female rats possess more V2R mRNA than male rats leading to increased urine osmolality and decreased urine volume in females compared with males - a finding which explains that clinical data according to which females are more prone to suffer from hyponatremia4. A recent study also showed that vasopressin interacts with V2R in sperm-cell membranes to enhance ion transport; over expression of the hormone reduced sperm motility, hence V2R also has an important part in male fertility8.

Applications may also work in :

Supplier :

Alomone Labs

Target :

V2R, AVPR V2, Antidiuretic hormone receptor, ADHR, Renal-type arginine vasopressin receptor, DIR3, Nephrogenic diabetes insipidus

Short Description :

A Rabbit Polyclonal Antibody to Vasopressin V2 Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Arginine vasopressin receptor 2. Anti-Vasopressin V2 Receptor (AVPR2) Antibody (#AVR-012) can be used in western blot analysis. It has been designed to recognize V2R from rat, mouse and human samples.

Negative Control :

BLP-VR012

Positive Control :

Synonyms :

V2R, AVPR V2, Antidiuretic hormone receptor, ADHR, Renal-type arginine vasopressin receptor, DIR3, Nephrogenic diabetes insipidus

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

AVPR2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage