Anti-Vasopressin V1B Receptor (AVPR1B) Antibody | Alomone Labs AVR-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Vasopressin V1B Receptor (AVPR1B) Antibody

catalog :

AVR-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Maruyama H, Taguchi A, Nishikawa Y, Guili C, Mikame M, Nameta M, et al. Medullary thick ascending limb impairment in the GlatmTg(CAG-A4GALT) Fabry model mice. FASEB J. 2018;32:4544-4559 pubmed publisher

image

image 1 :

Western blot analysis of rat lung (lanes 1 and 3) and mouse kidney (lanes 2 and 4) lysates: - 1,2. Anti-Vasopressin V1B Receptor (AVPR1B) Antibody (#AVR-011), (1:200).3,4. Anti-Vasopressin V1B Receptor (AVPR1B) Antibody, preincubated with Vasopressin V1B Receptor/AVPR1B Blocking Peptide (#BLP-VR011).

image 2 :

Peptide (C)RAKPRPAGSLKDLE corresponding to amino acid residues 399-412 of rat AVPR1B (AccessionP48974). Intracellular C-terminus.

image 3 :

Immunolocalization of p75NTRand TrkA in rat dorsal root ganglion - Immunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglia (DRG) sections using Anti-p75NGF Receptor (extracellular)-ATTO-488 Antibody(#ANT-007-AG) (1:60) andAnti-TrkA (extracellular)-ATTO-633 Antibody(#ANT-018-FR) (1:60). A. Sections were labeled with Anti-p75NGF Receptor (extracellular)-ATTO-488 Antibody(green). B. The same section was stained with Anti-TrkA (extracellular)-ATTO-633 Antibody (purple). C. Merge of the two images suggests extensive co-localization (arrow points at an example) of TrkA and p75NTR receptors in rat dorsal root ganglion. Cell nuclei were stained with DAPI (blue).

product information

CAT :

AVR-011

SKU :

AVR-011-CF_0.2 ml

Product Name :

Anti-Vasopressin V1B Receptor (AVPR1B) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P48974

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-VR011

Homology :

Mouse - 12/14 amino acid residues identical; human - not recommended for use with human sample

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RAKPRPAGSLKDLE, corresponding to amino acid residues 399-412 of rat AVPR1B

Product Page - Scientific background :

Vasopressin V1b receptor (also called V3R) is a G protein-coupled receptor (GPCR) of the Gq type, thus activating the phospholipase C signaling pathway upon activation. It is part of a family of GPCRs triggered by vasopressin that further include V1R, V2R, oxytocin receptors and purinergic receptors. While V1R and V3R are Gq GPCRs, V2R is coupled to the Gs protein and activates adenylate cyclase1,2.The V1b receptor has an intracellular C-terminal, seven transmembrane domains, and an extracellular N-tail; the transmembrane and extracellular domains are responsible for interacting with agonists and to signal for activation of the coupled Gq receptor1,2.The V1b receptor is mainly detected in the corticotrophs of the anterior pituitary gland in the brain where it regulates the release of adrenocorticotrophic hormone (ACTH) upon stimulation of vasopressin-corticotrophin-releasing hormone conjugates2.V1b KO mice show that the receptor is critical for the functionality of the hypothalamic-pituitary-adrenal axis, and demonstrate its importance in regulating glucose levels. Recent studies have also suggested that lactating rats treated with V1b antagonists, were less nurturing and similarly aggressive compared with untreated rats and that mice with reduced expression of V1b and V1a receptors have shown resistance to jet-lag1,3,4.

Applications may also work in :

Supplier :

Alomone Labs

Target :

V1bR, AVPR V1b, AVPR V3, Antidiuretic hormone receptor 1b, Arginine vasopressin receptor 1B, Vasopressin V3 receptor

Short Description :

A Rabbit Polyclonal Antibody to Vasopressin V1B Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Arginine vasopressin receptor 1B. Anti-Vasopressin V1B Receptor (AVPR1B) Antibody (#AVR-011) can be used in western blot analysis. It has been designed to recognize V1bR from rat and mouse samples. The antibody is not recommended for human samples.

Negative Control :

BLP-VR011

Positive Control :

Synonyms :

V1bR, AVPR V1b, AVPR V3, Antidiuretic hormone receptor 1b, Arginine vasopressin receptor 1B, Vasopressin V3 receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

AVPR1B

AB Specifictiy :

Not recommended for human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage