Guinea Pig Anti-Vasopressin V1A Receptor (AVPR1A) Antibody | Alomone Labs AVR-010-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea Pig Anti-Vasopressin V1A Receptor (AVPR1A) Antibody

catalog :

AVR-010-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):

image 2 :

Western blot analysis of rat kidney membranes (lanes 1 and 3) and mouse kidney membranes (lanes 2 and 4):

image 3 :

Expression of V1aR in rat medial septum.

product information

CAT :

AVR-010-GP

SKU :

AVR-010-GP-CF_0.2 ml

Product Name :

Guinea Pig Anti-Vasopressin V1A Receptor (AVPR1A) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P30560

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-VR010

Homology :

Mouse - identical; human - 10 out of 13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KFAKDDSDSMSRR, corresponding to amino acid residues 378-390 of rat AVPR1A

Product Page - Scientific background :

Vasopressin (AVP), the antidiuretic hormone, is a cyclic nonapeptide involved in the homeostasis of body fluid, blood volume, vascular tone, and blood pressure. AVP also belongs to the family of vasoactive and mitogenic peptides involved in physiological and pathological cell growth and differentiation1.AVP exerts its actions through binding to specific V1A, V1B, and V2, membrane receptors coupled to distinct second messengers2. V1 AVP receptor has the typical features of a G-protein coupled transmembrane receptor with seven putative hydrophobic domains, connected by three extracellular and three intracellular loops3.V1 receptors activate phospholipases A2, C, and D, resulting in the production of inositol 1,4,5-trisphosphate (IPS) and 1,Z-diacylglycerol (DAG), the mobilization of intracellular Ca2+, the influx of extracellular Ca2+, the activation of protein kinase C, and protein phosphorylation4. V1A AVP receptors have been shown by radioligand binding techniques to be present in vascular smooth muscle cells, hepatocytes, blood platelets, lymphocytes and monocytes, type 2 pneumocytes, adrenal cortex, brain (hippocampus septum et amygdalae), reproductive organs, retinal epithelium, renal mesangial cells, and the A10, A7r5,3T3, and WRK-1 cell lines4. V1A AVP receptors mediate cell contraction and proliferation, platelet aggregation, coagulation factor release, and glycogenolysis. V1B AVP receptors are located in the anterior pituitary where they stimulate ACTH release1.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

V1aR, AVPR V1a, Antidiuretic hormone receptor 1a, Vascular/hepatic-type arginine vasopressin receptor

Short Description :

A Guinea Pig Polyclonal Antibody to Vasopressin V1A Receptor (AVPR1A)

Long Description :

Guinea Pig Anti-Vasopressin V1A Receptor (AVPR1A) Antibody (#AVR-010-GP) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize V1aR from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Vasopressin V1A Receptor (AVPR1A) Antibody (#AVR-010) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Negative Control :

BLP-VR010

Positive Control :

Synonyms :

V1aR, AVPR V1a, Antidiuretic hormone receptor 1a, Vascular/hepatic-type arginine vasopressin receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

AVPR1A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage