Anti-PACAP Receptor 1 (PAC1) Antibody | Alomone Labs AVR-003 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-PACAP Receptor 1 (PAC1) Antibody

catalog :

AVR-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Minnig M, Park T, Echeveste Sanchez M, Cottone P, Sabino V. Viral-Mediated Knockdown of Nucleus Accumbens Shell PAC1 Receptor Promotes Excessive Alcohol Drinking in Alcohol-Preferring Rats. Front Behav Neurosci. 2021;15:787362 pubmed publisher
Niibori M, Kudo Y, Hayakawa T, Ikoma Seki K, Kawamata R, Sato A, et al. Mechanism of aspirin-induced inhibition on the secondary hyperalgesia in osteoarthritis model rats. Heliyon. 2020;6:e03963 pubmed publisher
Varodayan F, Minnig M, Steinman M, Oleata C, Riley M, Sabino V, et al. PACAP regulation of central amygdala GABAergic synapses is altered by restraint stress. Neuropharmacology. 2020;168:107752 pubmed publisher

image

image 1 :

Western blot analysis of mouse brain (lanes 1 and 4), rat brain (lanes 2 and 5) and rat cerebellum (lanes 3 and 6) lysates: - 1-3. Anti-PACAP Receptor 1 (PAC1) Antibody (#AVR-003), (1:500).4-6. Anti-PACAP Receptor 1 (PAC1) Antibody, preincubated with PACAP Receptor 1/PAC1 Blocking Peptide (#BLP-VR003).

image 2 :

Expression of PAC1 receptor in rat cerebellum - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-PACAP Receptor 1 (PAC1) Antibody (#AVR-003), (1:400), followed by donkey anti-rabbit-Cy3 antibody. PAC1 receptor staining (red) appears in Purkinje cells (arrows). Nuclei are stained with DAPI (blue).

image 3 :

Expression ofPAC1 receptor in rat cerebellum - Immunohistochemical staining of perfusion-fixed frozen rat brain sectionsusingAnti-PACAP Receptor 1 (PAC1) Antibody(#AVR-003) (1:400) followed by donkey anti-rabbit-Cy3 antibody.PAC1 receptor staining (red)appears in Purkinje cells (arrows). Nuclei are stained with DAPI (blue).

product information

CAT :

AVR-003

SKU :

AVR-003-CF_0.2 ml

Product Name :

Anti-PACAP Receptor 1 (PAC1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P32215

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-VR003

Homology :

Mouse - identical; human - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KVNRYFTMDFKHRH, corresponding to amino acid residues 474-487 of rat PAC1 receptor

Product Page - Scientific background :

Pituitary adenylate cyclase-activating polypeptide (PACAP, encoded by ADCYAP1) belongs a superfamily of structurally related peptide hormones, which include vasoactive intestinal peptide (VIP), glucagon, glucagon-like peptides, secretin, gastric inhibitory peptide and growth hormone-releasing hormone.PACAP acts through PAC1, VPAC1 and VPAC2, initiating multiple signaling pathways1. PAC1 is a class B member of the G-protein coupled receptors (GPCRs) superfamily. Class B receptors are differentiated from Class A by a relatively large N-terminal ectodomain that is crucial to ligand recognition2.PAC1R is mainly expressed on smooth muscle cells, neurons, endothelial cells and peritoneal macrophages3.PAC1R is associated with posttraumatic stress disorder (PTSD) symptoms and psychophysiological markers of fear and anxiety4.PAC1 receptor null mice demonstrate reduced anxiety-like behavior5. In addition, PAC1 overexpression can result in hydrocephalus- and schizophrenia-related neuropathology1.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

PACAP type I receptor, Pituitary adenylate cyclase-activating polypeptide type I receptor, PACAP-R1, ADCYAP1R1

Short Description :

A Rabbit Polyclonal Antibody to PACAP Type I Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat PACAP type I receptor. Anti-PACAP Receptor 1 (PAC1) Antibody (#AVR-003) can be used in western blot and immunohistochemistry applications. It has been designed to recognize PAC1 receptor from rat, mouse and human samples.

Negative Control :

BLP-VR003

Positive Control :

Synonyms :

PACAP type I receptor, Pituitary adenylate cyclase-activating polypeptide type I receptor, PACAP-R1, ADCYAP1R1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ADCYAP1R1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage