Anti-VPAC2 (VIPR2) (extracellular) Antibody | Alomone Labs AVR-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-VPAC2 (VIPR2) (extracellular) Antibody

catalog :

AVR-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5), and human Malme-3M melanoma cell lysate (lanes 3 and 6): - 1-3. Anti-VPAC2 (VIPR2) (extracellular) Antibody (#AVR-002), (1:600).4-6. Anti-VPAC2 (VIPR2) (extracellular) Antibody, preincubated with VPAC2/VIPR2 (extracellular) Blocking Peptide (#BLP-VR002).

image 2 :

Peptide CRFHLEIQEEETK corresponding to amino acid residues 25-37 of human VPAC2 (AccessionP41587). Extracellular N-terminus.

image 3 :

Cell surface detection of VPAC2 in live intact human Jurkat cell line: - ___Cells + goat-anti-rabbit-DyLight-488.___Cells +Anti-VPAC2 (VIPR2) (extracellular) Antibody(#AVR-002) (1:15) + goat-anti-rabbit-DyLight-488.

product information

CAT :

AVR-002

SKU :

AVR-002-CF_0.2 ml

Product Name :

Anti-VPAC2 (VIPR2) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P41587

Applications :

IC IF IFC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-VR002

Homology :

Mouse, rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CRFHLEIQEEETK, corresponding to amino acid residues 25-37 of human VPAC2

Product Page - Scientific background :

Vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating peptide (PACAP) are prominent neuropeptides that are structurally related. Two receptors have similarly high affinity for VIP and PACAP and have therefore been termed VIP and PACAP receptor 1 (VPAC1) and VIP and PACAP receptor 2 (VPAC2). VPAC2 (also known as vasoactive intestinal peptide receptor 2) belongs to the Class II family of G-protein coupled receptors1. A distinct feature of class II GPCRs is the presence of large N-terminal extracellular domains which contain 10 highly conserved amino acids including six cysteines, putative N terminal leader sequences and several potential N-glycosylation sites1.VPAC2 receptor is expressed in the uterus, prostate, smooth muscle of the gastrointestinal tract, seminal vesicles and skin, blood vessels and thymus2. VPAC2 is also expressed in various brain regions including the thalamus and the suprachiasmatic nuclei (SCN)3.VPAC2 plays important roles in the control of mammalian circadian rhythms in the SCN. Mice lacking VPAC2 show altered circadian rhythms in locomotor behavior, neuronal firing and clock gene expression4. Recent studies have also shown that duplication of the VPAC2 gene, and the resulting higher than normal VPAC2 signaling in patients confers a significant risk to schizophrenia5.

Applications may also work in :

IC IF IFC LCI WB

Supplier :

Alomone Labs

Target :

VIP and PACAP receptor 2, Vasoactive intestinal polypeptide receptor 2, Helodermin-preferring VIP receptor, Pituitary adenylate cyclase-activating polypeptide type III receptor, PACAP type III receptor, PACAP-R3

Short Description :

A Rabbit Polyclonal Antibody to VPAC2 Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of human VIP and PACAP receptor 2. Anti-VPAC2 (VIPR2) (extracellular) Antibody (#AVR-002) can be used in western blot analysis, immunocytochemistry and indirect flow cytometry applications. It has been designed to recognize VPAC2 from human, rat and mouse samples.

Negative Control :

BLP-VR002

Positive Control :

Synonyms :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

VIPR2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:600

more info or order :

Alomone Labs product webpage