Anti-VPAC1 (VIPR1) (extracellular) Antibody | Alomone Labs AVR-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-VPAC1 (VIPR1) (extracellular) Antibody

catalog :

AVR-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Kim D, Yoon Y, Choi D, Kim J, Lim J. 3D Organoid Culture From Adult Salivary Gland Tissues as an ex vivo Modeling of Salivary Gland Morphogenesis. Front Cell Dev Biol. 2021;9:698292 pubmed publisher
Ivic I, Balasko M, Fulop B, Hashimoto H, Toth G, Tamas A, et al. VPAC1 receptors play a dominant role in PACAP-induced vasorelaxation in female mice. PLoS ONE. 2019;14:e0211433 pubmed publisher
Ivic I, Fulop B, Juhasz T, Reglodi D, Toth G, Hashimoto H, et al. Backup Mechanisms Maintain PACAP/VIP-Induced Arterial Relaxations in Pituitary Adenylate Cyclase-Activating Polypeptide-Deficient Mice. J Vasc Res. 2017;54:180-192 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human Jurkat T cell leukemia cell lysate (lanes 3 and 6): - 1-3. Anti-VPAC1 (VIPR1) (extracellular) Antibody (#AVR-001), (1:400).4-6. Anti-VPAC1 (VIPR1) (extracellular) Antibody, preincubated with VPAC1/VIPR1 (extracellular) Blocking Peptide (#BLP-VR001).

image 2 :

Expression of VPAC1 in rat amygdala - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-VPAC1 (VIPR1) (extracellular) Antibody (#AVR-001), (1:100). A. VPAC1 staining (red) is apparent in basolateral amygdala neurons (horizontal arrow). B. Cell nuclei in the same section are stained with DAPI (Blue). C. Merge of the two images.

image 3 :

Cell surface detection of VPAC1 in live intact Jurkat (human T cell leukemia cells) cell line: - ___Cells + goat-anti-rabbit-DyLight-488.___Cells +Anti-VPAC1 (VIPR1) (extracellular) Antibody(#AVR-001) (1:20) + goat-anti-rabbit-DyLight-488.

product information

CAT :

AVR-001

SKU :

AVR-001-CF_0.2 ml

Product Name :

Anti-VPAC1 (VIPR1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P32241

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-VR001

Homology :

Human - 13/14 amino acid residues identical; rat, mouse - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)EEAQLENETIG(S)SK, corresponding to amino acid residues 52-65 of human VPAC1

Product Page - Scientific background :

Vasointestinal peptide (VIP) and pituitary adenylate cyclase-activating peptide (PACAP) belong to the glucagon hormone superfamily, which includes secretin, growth hormone-releasing hormone (GHRH), glucagon, glucagon-like peptides 1 and 2 (GLP-1 and GLP-2), peptide histidine methionine (PHM), and glucose-dependent insulinotropic polypeptide (GIP)1. PACAP and VIP effects have been described in the digestive tract, cardiovascular system, airways, reproductive system, immune system, endocrine glands, and brain2. VIP and PACAP share a common G-protein coupled receptor, VPAC13.VPAC1 is a membrane-associated protein and shares significant homology with members of the G-protein coupled class B receptor family, the most important of which is the presence of large N-terminal extracellular domains which contain 10 highly conserved amino acids including six cysteines, putative N-terminal leader sequences and several potential N-glycosylation sites4. In the CNS, VPAC1 receptors are abundantly localized in piriform cortex, cerebral cortex, suprachiasmatic nucleus, hippocampus, and pineal gland5. In peripheral tissues, VPAC1 receptors have been found in breast, kidney, liver, lung, prostate, spleen, and mucosa of the gastrointestinal tract6. VPAC1 mediates a large array of VIP and PACAP actions on exocrine secretion, hormones release, muscle relaxation, metabolism, fetus growth, tumor cells and embryonic brain development7.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

VIP and PACAP receptor 1, Vasoactive intestinal polypeptide receptor 1, Pituitary adenylate cyclase-activating polypeptide type II receptor, PACAP type II receptor, PACAP-R2

Short Description :

A Rabbit Polyclonal Antibody to VPAC1 Receptor

Long Description :

Anti-VPAC1 (VIPR1) (extracellular) Antibody (#AVR-001) is a highly specific antibody directed against an epitope of human VIP and PACAP receptor 1. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, live cell imaging, and indirect flow cytometry applications. It has been designed to recognize VPAC1 from mouse, rat, and human samples.

Negative Control :

BLP-VR001

Positive Control :

Synonyms :

VIP and PACAP receptor 1, Vasoactive intestinal polypeptide receptor 1, Pituitary adenylate cyclase-activating polypeptide type II receptor, PACAP type II receptor, PACAP-R2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

VIPR1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage