Anti-AXL (extracellular) Antibody | Alomone Labs ATR-032 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-AXL (extracellular) Antibody

catalog :

ATR-032

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and mouse lung lysate (lanes 3 and 6): - 1-3. Anti-AXL (extracellular) Antibody (#ATR-032), (1:200). 4-6. Anti-AXL (extracellular) Antibody, preincubated with AXL (extracellular) Blocking Peptide (BLP-TR032).

image 2 :

Expression of AXL in rat parietal cortex. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-AXL (extracellular) Antibody (#ATR-032), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. AXL immunoreactivity (green) appears in neuron outlines (arrows). B. Pre-incubation of the antibody with AXL (extracellular) Blocking Peptide (BLP-TR032), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ATR-032

SKU :

ATR-032-CF_0.2 ml

Product Name :

Anti-AXL (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P30530

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-TR032

Homology :

Mouse, Rat - 14 out of 15 amino acids residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-term.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)RLAYQGQDTPEVLMD, corresponding to amino acid residues 368 - 382 of human AXL

Product Page - Scientific background :

AXL, also known as Tyrosine Kinase Receptor UFO and Tyro7, is a member of the receptor tyrosine kinase TAM family, that includes the receptors Tyro3, AXL and MERTK 1,2.The TAM family receptors are widely expressed in normal cells and tissues, such as monocytes, platelets, endothelial cells, brain and heart, where they regulate cell survival, non-inflammatory clearance of apoptotic cells by phagocytic cells, natural killer cell differentiation, platelet aggregation, and more 1,2.The TAM family receptors show structural similarities with other tyrosine kinase receptors, that is, an extracellular N-terminal domain containing two immunoglobulin-like and two fibronectin 3 domains, followed by a hydrophobic single pass transmembrane domain, and a cytoplasmic C-terminal tail containing a tyrosine kinase domain1,2.Axl and the other TAM receptors can be activated via their ligands, growth arrest specific 6 protein (Gas6) and Protein S (Pros1), which are members of the family of vitamin K-dependent proteins 3.Both AXL receptor and its high affinity ligand Gas6, are key regulators of immune cell activation, and have been shown to be expressed in cancer cells where they promote survival and invasion and contribute to resistance to various therapies 3,4,5. AXL expression is linked to increased risk of metastasis and poor survival in a variety of solid cancers including breast cancer, non-small cell lung carcinoma, ovarian cancer, and clear cell renal carcinoma 3,4. AXL activation in cancer cells and various stromal cells also results in tumor microenvironment deregulation, leading to modulation of angiogenesis, fibrosis, immune response and hypoxia 3,4.Based on these findings, AXL has been identified as a critical therapeutic target for solid cancers, and indeed several therapeutic modalities including small molecules and monoclonal antibodies, are currently being developed 2, 4.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

AXL Receptor Tyrosine Kinase, Tyrosine-Protein Kinase Receptor UFO, AXL Oncogene, Tyro7, UFO

Short Description :

A Rabbit Polyclonal antibody to AXL (extracellular)

Long Description :

Anti-AXL (extracellular) Antibody (#ATR-032) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize AXL from mouse, rat and human samples.

Negative Control :

BLP-TR032

Positive Control :

Synonyms :

AXL Receptor Tyrosine Kinase, Tyrosine-Protein Kinase Receptor UFO, AXL Oncogene, Tyro7, UFO

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

AXL

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage