Anti-Trace Amine Receptor 1 (TAAR1) Antibody | Alomone Labs ATR-021 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Trace Amine Receptor 1 (TAAR1) Antibody

catalog :

ATR-021

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat liver (lane 1 and 4), mouse kidney (lanes 2 and 5) and human brain glioblastoma (U-87) cell (lanes 3 and 6) lysates: - 1-3. Anti-Trace Amine Receptor 1 (TAAR1) Antibody (#ATR-021), (1:200).4-6. Anti-Trace Amine Receptor 1 (TAAR1) Antibody, preincubated with Trace Amine Receptor 1/TAAR1 Blocking Peptide (#BLP-TR021).

image 2 :

Western blot analysis of rat liver (lane 1 and 4) mouse kidney (lanes 2 and 5) and human brain glioblastoma (U-87) cell (lanes 3 and 6) lysates: - 1-3.Anti-Trace Amine Receptor 1 (TAAR1) Antibody(#ATR-021) (1:200).4-6. Anti-Trace Amine Receptor 1 (TAAR1) Antibody preincubated with the negative control antigen.

image 3 :

Cell surface detection ofnAChR?7 in live intact human Jurkat T cell leukemia cell line: - ___ Cells alone.___Cells + rabbit-IgG-Alexa-647.___Cells +Anti-Nicotinic Acetylcholine Receptor ?7 (CHRNA7) (extracellular)-ATTO-633Antibody (#ANC-007-FR) (1:10).

product information

CAT :

ATR-021

SKU :

ATR-021-CF_0.2 ml

Product Name :

Anti-Trace Amine Receptor 1 (TAAR1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q923Y9

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-TR021

Homology :

Mouse, human - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KMVLFGKIFQKDS, corresponding to amino acid residues 313-325 of rat TAAR1

Product Page - Scientific background :

Trace amines are compounds, such as p-tyramine and tryptamine, found in the body in trace amounts. These substances were believed to mainly modulate aminergic pathways. However, they were found to activate Trace amino associated receptors (TAARs), members of the G-protein coupled receptor (GPCRs) superfamily1.Trace amine receptor 1 (TAAR-1) is part of the Rhodopsin family of GPCRs. Five other putative genes are thought to encode TAAR receptors. This receptor has a seven transmembrane domain with short extracellular N- and intracellular C- terminal domains.TAAR-1 couples Gs, thus leading to an increase in intracellular cAMP levels. Its signaling might be enhanced by monoamine transporters, and activation of TAAR-1 may also affect monoamine transport function. The receptor also inhibits PKA and PKC mediated dopamine uptake and PKC mediated dopamine efflux2. It is also possible that it forms heterodimers with dopamine receptors, thus influencing dopamine transmission3.In humans, this receptor is mainly expressed in the stomach but is also detected in the lungs, small intestine and many cerebral and cerebellar tissues including the amygdala, hypothalamus and others4.In experiments conducted in mice it was shown that TAAR-1 knockout mice display increased stimulation effects of d-amphetamine, greater levels of extracellular monoamine and hyperactivity. These effects are related to clinical conditions such as ADHD, psychosis and other psychiatric problems5. Additional studies on TAAR family receptors may be helpful in finding cutting edge treatment.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Trace amine-associated receptor 1, TaR-1, TA1, TRAR1

Short Description :

A Rabbit Polyclonal Antibody to Trace Amine Receptor 1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Trace amine-associated receptor 1. Anti-Trace Amine Receptor 1 (TAAR1) Antibody (#ATR-021) can be used in western blot analysis. It has been designed to recognize TAAR1 from rat, mouse and human samples.

Negative Control :

BLP-TR021

Positive Control :

Synonyms :

Trace amine-associated receptor 1, TaR-1, TA1, TRAR1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TAAR1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage