Anti-Taste Receptor Type 1 Member 3 (TAS1R3) (extracellular) Antibody

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Taste Receptor Type 1 Member 3 (TAS1R3) (extracellular) Antibody

catalog :

ATR-016

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):

image 2 :

Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human K562 chronic myelogenous leukemia cell line lysate (lanes 2 and 4):

image 3 :

Expression of TAS1R3 in rat hypothalamus

product information

CAT :

ATR-016

SKU :

ATR-016-CF_0.2 ml

Product Name :

Anti-Taste Receptor Type 1 Member 3 (TAS1R3) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q925D8

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-TR016

Homology :

Rat – identical; Human - 12/14 identical.

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

CKDGQVRRVKGFHS, corresponding to amino acid residues 508 - 521 of mouse TAS1R3

Product Page - Scientific background :

Taste receptor type 1 member 3 (TAS1R3), commonly known as T1R3, is a key component in the detection of sweet and umami tastes. TAS1R3 is part of the Class C G-protein-coupled receptor (GPCR) family and functions as a heterodimer with TAS1R1 or TAS1R2 to mediate umami and sweet taste perception, respectively. Structurally, members of this family share a large extracellular ligand-binding domain, seven transmembrane domains, and intracellular signaling regions1,3. TAS1R3 is localized primarily on the cell membrane of taste receptor cells within taste buds in the tongue’s fungiform, foliate, and circumvallate papillae3,5. TAS1R3 expression extends to the gastrointestinal tract and specific enteroendocrine cells, linking its activation to nutrient sensing and gut hormone secretion2,4. Emerging evidence suggests that TAS1R3 is expressed in the hypothalamus, particularly in the paraventricular and arcuate nuclei, where it could play a role in integrating peripheral nutrient signals with neuroendocrine and behavioral responses6. Upon binding of sugars, amino acids, or fatty acids, TAS1R3 activates downstream signaling via the Gα-gustducin and phospholipase Cβ2 pathways, leading to calcium mobilization and neurotransmitter release3,5. TAS1R3-mediated signaling plays a significant role in nutrient detection, appetite regulation, and gut-brain axis communication. Dysfunction or altered TAS1R3 expression is implicated in metabolic disorders such as obesity, diabetes, and intestinal inflammation4.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Sweet Taste Receptor T1R3, T1R3

Short Description :

A Rabbit Polyclonal Antibody to Taste Receptor Type 1 Member 3

Long Description :

Anti-Taste Receptor Type 1 Member 3 (TAS1R3) (extracellular) Antibody (#ATR-016) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and flow cytometry applications. It has been designed to recognize TAS1R3 from mouse, rat and human samples.

Negative Control :

BLP-TR016

Positive Control :

Synonyms :

Sweet Taste Receptor T1R3, T1R3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TAS1R3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Standard quality control of each lot :

Western blot analysis.

Antigen preadsorption control :

2 µg peptide per 1 µg antibody

more info or order :

Alomone Labs product webpage