Anti-TSH Receptor (TSHR) (extracellular) Antibody | Alomone Labs ATR-006 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TSH Receptor (TSHR) (extracellular) Antibody

catalog :

ATR-006

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Agarwal S, Koh K, Tardi N, Chen C, Dande R, WerneckdeCastro J, et al. Deiodinase-3 is a thyrostat to regulate podocyte homeostasis. EBioMedicine. 2021;72:103617 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-TSH Receptor (TSHR) (extracellular) Antibody (#ATR-006), (1:200).3,4. Anti-TSH Receptor (TSHR) (extracellular) Antibody, preincubated with TSH Receptor/TSHR (extracellular) Blocking Peptide (#BLP-TR006).

image 2 :

Expression of TSH Receptor in mouse hippocampus. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-TSH Receptor (TSHR) (extracellular) Antibody (#ATR-006), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal CA3 region, showed immunoreactivity (green) in the pyramidal layer (P, arrows) and in mossy fiber terminal region (MF). B. Pre-incubation of the antibody with TSH Receptor (TSHR) (extracellular) Blocking Peptide (BLP-TR006), suppressed staining. Cell nuclei are stained with DAPI (blue).

image 3 :

Peptide (C)KLPKNINGTDPIQK corresponding to amino acid residues 487-500 of human ANO6 (Accession Q4KMQ2). 2nd extracellular loop.

product information

CAT :

ATR-006

SKU :

ATR-006-CF_0.2 ml

Product Name :

Anti-TSH Receptor (TSHR) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P21463

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-TR006

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

ETLQAFDSHYDYTVC, corresponding to amino acid residues 376-390 of rat TSHR

Product Page - Scientific background :

Thyroid stimulating hormone (TSH), a glycoprotein hormone composed of α and β chains, is produced by thyrotrope cells of the anterior pituitary1. The TSH receptor (TSHR) plays a prominent role in thyroid physiology and disease. The established biological function of TSHR in the thyroid gland is to regulate synthesis and secretion of thyroid hormones from follicular thyroid cells; it also plays an important role in controlling the growth and development of the thyroid gland2.TSHR is a G-protein coupled receptor and shares the classic structure of the serpentine receptor family (seven membrane spanning segments, an extracellular N-terminus and an intracellular carboxy terminal)3.TSHR is primarily expressed in the epithelial cells of the thyroid follicles, but TSHR mRNA and protein have been detected in a variety of cell types including brain, testes, kidney, heart, bone, thymus, lymphocytes, adipose tissue and fibroblasts4.Chronic stimulation of the TSHR leads to over activation of the cAMP pathway that in turn causes thyroid hyperplasia and hyperthyroidism. This process occurs in Graves' disease (GD) in which thyroid stimulating antibodies (TSAB) bind the receptor and mimic the action of TSH5.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Thyrotropin receptor, Thyroid-stimulating hormone receptor, TSH-R

Short Description :

A Rabbit Polyclonal Antibody to TSH Receptor

Long Description :

Anti-TSH Receptor (TSHR) (extracellular) Antibody (#ATR-006) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and thus is ideal for detecting the receptor in living cells. It has been designed to recognize TSHR from human, rat, and mouse samples.

Negative Control :

BLP-TR006

Positive Control :

Synonyms :

Thyrotropin receptor, Thyroid-stimulating hormone receptor, TSH-R

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TSHR

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage