Anti-TRHR1 Antibody | Alomone Labs ATR-005 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRHR1 Antibody

catalog :

ATR-005

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 4), human Jurkat acute T cell leukemia cell (lanes 2 and 5) and mouse MS1 pancreas cell (lanes 3 and 6) lysates: - 1-3. Anti-TRHR1 Antibody (#ATR-005), (1:200).4-6. Anti-TRHR1 Antibody, preincubated with TRHR1 Blocking Peptide (#BLP-TR005).

image 2 :

Peptide CKQKPTEKAANYS corresponding to amino acid residues 336-349 of rat Thyrotropin-releasing hormone receptor 1 (AccessionQ01717). Intracellular C-terminus.

image 3 :

Cell surface detection of P2RX4 in live intact human THP-1 monocytic leukemia cells: - ___Cells.___Cells + rabbit IgG isotype control-FITC.___Cells + Anti-P2X4 Receptor (extracellular)-FITC Antibody (#APR-024-F) (2.5 g).

product information

CAT :

ATR-005

SKU :

ATR-005-CF_0.2 ml

Product Name :

Anti-TRHR1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q01717

Applications :

IC IF WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-TR005

Homology :

Mouse - identical; human -12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CKQKPTEKAANYS, corresponding to amino acid residues 336-349 of rat Thyrotropin-releasing hormone receptor 1

Product Page - Scientific background :

Thyrotropin-releasing hormone (TRH) is a widely distributed tripeptide which acts as a hormone, a paracrine regulatory factor and a neurotransmitter/neuromodulator. It is expressed in many parts of the brain as well as in various peripheral tissues1,2.In the hypothalamus, TRH is secreted from the paraventricular nucleus (VPN) by TRH neurons prior to a post-translational processing from its precursor, pro-TRH. A key player in the regulation of the hypothalamus-pituitary-thyroid (HPT) axis, TRH is released into the hypophyseal portal circulation of the median eminence and reaches the pituitary, where it binds to TRH receptors, thereby stimulating the secretion of tyrothropin (thyroid-stimulating hormone; TSH). TSH, in turn, affects the thyroid to produce T4 which is subsequently converted into its biologically-active analog, T3 - a negative mediator of TRH. In the tanycytes, ependymal cells of glial origin located in close proximity to the VPN, T4 is converted to T3 by the enzyme deiodinase II (dio2) - a counterpart to deiodinase III (dio3) which conversely inactivates the above mentioned thyroid hormones, thus indirectly promoting the production of TRHs3,4.TRH receptor 1 (TRH-R1), the only identified TRH receptor in humans, is a G-protein coupled receptor (GPCR) abundant in the anterior pituitary, neuroendocrine system, autonomic nervous system and the visceral regions of the brain2,5. Like all members of GPCRs, the TRH receptor has seven transmembrane domains, and extracellular N-terminus and intracellular C-terminal tail. TRH receptor couples Gq upon activation which in turn leads to the activation of phospholipase C5.From a behavioral aspect, it was shown that TRH-coupled TRH-R1 increases cognitive arousal by directly exciting the sleep/wake system in the lateral hypothalamic area, and inhibits seizure activity in the hippocampus by facilitating GABA release. Conversely, its deficiency, as observed in mutant model mice, is linked to growth retardation, depression, and anxiety-like behavior1,6,7.

Applications may also work in :

IC IF WB

Supplier :

Alomone Labs

Target :

Thyrotropin-releasing hormone receptor 1, TRH1 receptor, TRHR, Thyroliberin receptor

Short Description :

A Rabbit Polyclonal Antibody to Thyrotropin-Releasing Hormone Receptor 1

Long Description :

Anti-TRHR1 Antibody (#ATR-005) is a highly specific antibody directed against an epitope of the rat Thyrotropin-releasing hormone receptor 1. The antibody can be used in western blot and immunocytochemistry applications. It has been designed to recognize TRH-R from rat, mouse, and human samples.

Negative Control :

BLP-TR005

Positive Control :

Synonyms :

Thyrotropin-releasing hormone receptor 1, TRH1 receptor, TRHR, Thyroliberin receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TRHR

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage