Anti-Neurokinin 2 Receptor (NK2R) Antibody | Alomone Labs ATR-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Neurokinin 2 Receptor (NK2R) Antibody

catalog :

ATR-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Delvalle N, Dharshika C, Morales Soto W, Fried D, Gaudette L, Gulbransen B. Communication Between Enteric Neurons, Glia, and Nociceptors Underlies the Effects of Tachykinins on Neuroinflammation. Cell Mol Gastroenterol Hepatol. 2018;6:321-344 pubmed publisher
Yuan M, Ma M, Wang T, Feng Y, Chen P, He C, et al. Direct activation of tachykinin receptors within baroreflex afferent pathway and neurocontrol of blood pressure regulation. CNS Neurosci Ther. 2019;25:123-135 pubmed publisher

image

image 1 :

Western blot analysis of rat hippocampus (lanes 1 and 3) and rat uterus (lanes 2 and 4): - 1,2. Anti-Neurokinin 2 Receptor (NK2R) Antibody (#ATR-002), (1:200).3,4. Anti-Neurokinin 2 Receptor (NK2R) Antibody, preincubated with Neurokinin 2 Receptor/NK2R Blocking Peptide (#BLP-TR002).

image 2 :

Western blot analysis of rat hippocampus (lanes 1 and 3) and rat uterus (lanes 2 and 4): - 12.Anti-Neurokinin 2 Receptor (NK2R) Antibody(#ATR-002) (1:200).34. Anti-Neurokinin 2 Receptor (NK2R) Antibodypreincubated with the negative control antigen.

image 3 :

Cell surface detection of P2RX4 in live intact mouse BV-2 microglia cells: - ___Cells.___Cells + rabbit IgG isotype control-FITC.___Cells + Anti-P2X4 Receptor (extracellular)-FITC Antibody (#APR-024-F) (2.5 g).

product information

CAT :

ATR-002

SKU :

ATR-002-CF_0.2 ml

Product Name :

Anti-Neurokinin 2 Receptor (NK2R) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P16610

Applications :

IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-TR002

Homology :

Mouse - identical; human - 13/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CHTKETLFMTGDMTHSE, corresponding to amino acid residues 351-367 of rat NK2 receptor

Product Page - Scientific background :

Substance P (SP), Neurokinin A (NKA) and Neurokinin B (NKB) are all peptides belonging to the Tachykinin protein family. These three peptides, which demonstrate quite a heterogeneity in their distribution, exert their effect via three receptors: Neurokinin 1-3 receptors, members of the G-protein coupled receptor superfamily. However, Neurokinin 1 (NK1) receptor preferentially binds Substance P, Neurokinin 2 (NK2) receptor to NKA, and Neurokinin 3 (NK3) receptor to NKB1.Neurokinin receptors are distinguished by their seven transmembrane domains, an extracellular N-terminus and a cytosolic C-terminal. An unusual property of these receptors is the presence of introns as part of their structural organization1-3. Tachykinin receptors undergo alternative splicing. For example, NK1 receptor is detected with different C-terminal lengths. The longer receptor isoform is found in the brain whereas the truncated form is mostly detected in the periphery1,4.Due to the broad expression profile of tachykinin peptides, their respective receptors are also expressed in a similar fashion. NK1 receptor is widely expressed in neurons, endothelial cells, muscle and immune system cells. NK2 receptor is broadly expressed in the periphery and its expression in the brain is quite restricted. NK3 receptor on the other hand is largely expressed in the central nervous system and is also detected in the uterus, skeletal muscle, lung and liver1.Neurokinin receptors have been found in many pathophysiological indications and have therefore become targets for the development of pharmacological compounds. Such indications include cancer, psychological disorders, migraine and various inflammations, just to name a few5-8.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

NK2 receptor, Neurokinin A receptor, Tachykinin receptor 2, TACR2, Substance-K receptor, SKR

Short Description :

A Rabbit Polyclonal Antibody to Neurokinin 2 Receptor

Long Description :

Anti-Neurokinin 2 Receptor (NK2R) Antibody (#ATR-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NK2 receptor from rat, mouse, and human samples.

Negative Control :

BLP-TR002

Positive Control :

Synonyms :

NK2 receptor, Neurokinin A receptor, Tachykinin receptor 2, TACR2, Substance-K receptor, SKR

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TACR2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage