Anti-TMC6 (EVER1) Antibody | Alomone Labs ATC-006 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TMC6 (EVER1) Antibody

catalog :

ATC-006

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat prostate (lanes 1 and 4), testis (lanes 2 and 5) and placenta (lanes 3 and 6): - 1-3. Anti-TMC6 (EVER1) Antibody (#ATC-006), (1:800).4-6. Anti-TMC6 (EVER1) Antibody, preincubated with TMC6/EVER1 Blocking Peptide (#BLP-TC006).

image 2 :

Expression of Orai1 in rat RBL cells - Cell surface detection ofOrai1 in intact living rat basophilic leukemia (RBL) cells using Anti-Orai1 (extracellular)-ATTO-488Antibody (#ACC-062-AG) (1:100). A. Extracellular staining of RBL cells (green). B. Merge of A and live view of the cells.

product information

CAT :

ATC-006

SKU :

ATC-006-CF_0.2 ml

Product Name :

Anti-TMC6 (EVER1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q7Z403

Applications :

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-TC006

Homology :

Rat, mouse - 13/14 amino acid residues are identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, cytoplasm

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CGPFRTLDTMYEAGR, corresponding to amino acid residues 681-695 of human TMC6

Product Page - Scientific background :

The EVER gene belongs to a larger family of proteins referred to as the transmembrane channel-like protein (TMC) family1. The EVER1/TMC6 integral membrane protein, is located in the endoplasmic reticulum (ER) of keratinocytes, where it forms a complex with zinc transporter-1 (ZnT-1), a membrane protein responsible for zinc efflux and resistance to zinc toxicity, and thereby controlling the cellular zinc balance2. The EVER1 protein is predicted to have 10 transmembrane domains and 2 leucine zipper motifs1.The EVER1 gene encodes 2 alternatively spliced proteins, one 805 amino acids long and the other 454 amino acids, in the same open reading frame. The EVER genes are thought to be transcribed in numerous tissues including the skin1. Mutations in the TMC6 are implicated in the development of the rare autosomal recessive disease Epidermodysplasia Verruciformis (EV)3. EV is characterized by extreme susceptibility to infection by cutaneous human papillomaviruses (HPV) of the beta genus. The TMC6 genes may be involved in controlling HPV gene expression and replication in epidermal keratinocytes or they may directly affect innate and adaptive immune responses which control the clearance of HPV-infected keratinocytes in EV cases4. A recent report indicated an association between genetic variation in the TMC6 region and development of cervical cancer (CxCa)5.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Transmembrane channel-like protein 6, Epidermodysplasia verruciformis protein 1, Protein LAK-4, LAK4P, EVIN1, EV1

Short Description :

A Rabbit Polyclonal Antibody to TMC6 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human Transmembrane channel-like protein 6. Anti-TMC6 (EVER1) Antibody (#ATC-006) can be used in western blot analysis. It has been designed to TMC6 from rat, mouse and human samples.

Negative Control :

BLP-TC006

Positive Control :

Synonyms :

Transmembrane channel-like protein 6, Epidermodysplasia verruciformis protein 1, Protein LAK-4, LAK4P, EVIN1, EV1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TMC6

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:800

more info or order :

Alomone Labs product webpage