Anti-TRIC-A (TMEM38A) Antibody | Alomone Labs ATC-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TRIC-A (TMEM38A) Antibody

catalog :

ATC-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Shrestha N, Bacsa B, Ong H, Scheruebel S, Bischof H, Malli R, et al. TRIC-A shapes oscillatory Ca2+ signals by interaction with STIM1/Orai1 complexes. PLoS Biol. 2020;18:e3000700 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 4), and mouse brain membrane (lanes 2 and 5) and skeletal muscle (lanes 3 and 6): - 1-3. Anti-TRIC-A (TMEM38A) Antibody (#ATC-002), (1:200).4-6. Anti-TRIC-A (TMEM38A) Antibody, preincubated with TRIC-A/TMEM38A Blocking Peptide (#BLP-TC002).

image 2 :

Expression of TRIC-A in rat skeletal muscle - Immunohistochemical staining of paraffin-embedded rat skeletal muscle sections using Anti-TRIC-A (TMEM38A) Antibody (#ATC-002), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. TRIC-A labeling (red) appears in the edges of the muscle fibers, where the endomysium is present. B. Nuclear staining using DAPI as the counterstain. C. Merged images of A and B.

image 3 :

Expression of TRIC-A in mouse muscle myoblast (C2C12) cell line - Immunocytochemical staining of mouse paraformaldehyde-fixed and permeabilized muscle myoblast (C2C12) cell line. A. Cells were stained withAnti-TRIC-A (TMEM38A) Antibody(#ATC-002) (1:200) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Nuclear staining using DAPI as the counterstain (blue). C. Merged images of panels A and B.

product information

CAT :

ATC-002

SKU :

ATC-002-CF_0.2 ml

Product Name :

Anti-TRIC-A (TMEM38A) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

A6ZIQ8

Applications :

IC IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-TC002

Homology :

Mouse - identical; human - will not recognize human TRIC-A

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DNHGAPHGMGLGTQHS, corresponding to amino acid residues 259-274 of rat TRIC-A

Product Page - Scientific background :

Intracellular Ca2+ levels are important in proper cellular functions and have prominent roles in various cell signaling pathways and are crucial for muscle contractions. Indeed, an important step leading to muscle contraction is the massive release of Ca2+ ions from the endoplasmic/sarcoplasmic reticulum (ER/SR) to the cytosol. A battery of results suggest that specific K+ channels are important to counteract the Ca2+ outflow in order to neutralize the negative potential created by the movement of Ca2+ ions. It is believed that TRIC channels are responsible for neutralizing this negative potential1,2.Trimeric intracellular cation-specific (TRIC) channels are critical for proper management of intracellular stores. TRIC-A and TRIC-B both belong to this family and are both permeable to monovalent ions with a preference for K+ 3. Both channels are localized to the ER/SR membrane. Each TRIC subunit contains three transmembrane domains, a cytoplasmic C-terminus and a luminal N-terminus. Functional entities are formed by homotrimerizarion4. The activity of TRIC-A is regulated by voltage whereas that of TRIC-B can be regulated by different mechanisms3.Knockout studies of these channels have shown that TRIC-A knockout mice are viable while those of TRIC-B die at the neonatal stage5.TRIC-A is mostly expressed in excitable tissues like the brain and muscle while TRIC-B is ubiquitously expressed.

Applications may also work in :

IC IHC WB

Supplier :

Alomone Labs

Target :

Trimeric intracellular cation channel type A, 27 kDa sarcoplasmic reticulum protein, Transmembrane protein 38A

Short Description :

A Rabbit Polyclonal Antibody to TRIC-A Channel

Long Description :

Anti-TRIC-A (TMEM38A) Antibody (#ATC-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize TRIC-A from rat and mouse samples. The antibody is not suited to recognize TRIC-A from human samples.

Negative Control :

BLP-TC002

Positive Control :

Synonyms :

Trimeric intracellular cation channel type A, 27 kDa sarcoplasmic reticulum protein, Transmembrane protein 38A

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TMEM38A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage