Anti-SVCT2/SLC23A2 (extracellular) Antibody | Alomone Labs AST-022 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SVCT2/SLC23A2 (extracellular) Antibody

catalog :

AST-022

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: - 1,2. Anti-SVCT2/SLC23A2 (extracellular) Antibody (#AST-022), (1:200).3,4. Anti-SVCT2/SLC23A2 (extracellular) Antibody, preincubated with SVCT2/SLC23A2 (extracellular) Blocking Peptide (#BLP-ST022).

image 2 :

Expression of SVCT2 in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SVCT2/SLC23A2 (extracellular) Antibody (#AST-022), (1:200), followed by goat anti-rabbit-AlexaFluor-488. SVCT2 staining (green) in CA1 hippocampal region is detected in axonal processes (arrows), with the highest density surrounding the pyramidal layer (P). Cell nuclei are stained with DAPI (blue).

product information

CAT :

AST-022

SKU :

AST-022-CF_0.2 ml

Product Name :

Anti-SVCT2/SLC23A2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9EPR4

Applications :

IF IFC IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-ST022

Homology :

Rat - identical; human - 11/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)EITVANGTAELLEH, corresponding to amino acid residues 191-204 of mouse SVCT2

Product Page - Scientific background :

Vitamin C, or ascorbic acid, is an important antioxidant. It also participates in numerous cellular functions such as peptide amidation, myelin formation, synaptic potentiation, and protection against glutamate toxicity. Although it circulates in the plasma in micromolar concentrations, it reaches millimolar concentrations in most tissues. These high concentrations are thought to be generated and maintained by SVCT2, a Na+-dependent transporter1.SVCT2 belongs to the SLC23 family of nucleobase-ascorbate transporters (NAT), that consists of SLCT1, SLCT2 and SLCT3 transporters2. The NAT family shares a structure that contains fourteen transmembrane segments that are organized into a pair of "7 + 7" inverted repeats. The intertwined repeats constitute two distinctive domains, namely the core domain and the gate domain2.Neurons in the central nervous system (CNS) contain some of the highest ascorbic acid concentrations in mammalian tissues, and therefore targeted deletion of SVCT2 in mice causes widespread cerebral hemorrhage and death on post-natal day one. SCVT2 is also related to neurodegenerative diseases such as Alzheimer's, Parkinson's, and Huntington's. The expression of SVCT2 is relatively widespread, where it serves to either deliver ascorbic acid to tissues with high demand of vitamin C for enzymatic reactions or to protect metabolically highly active cells or protect specialized tissues from oxidative stress3.

Applications may also work in :

IF IFC IHC WB

Supplier :

Alomone Labs

Target :

Sodium-dependent vitamin C transporter 2, Na+/L-Ascorbic acid transporter 2, Solute carrier family 23 member 2

Short Description :

A Rabbit Polyclonal Antibody to SVCT2 Transporter

Long Description :

Anti-SVCT2/SLC23A2 (extracellular) Antibody (#AST-022) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize SVCT2 from rat, mouse, and human samples.

Negative Control :

BLP-ST022

Positive Control :

Synonyms :

Sodium-dependent vitamin C transporter 2, Na+/L-Ascorbic acid transporter 2, Solute carrier family 23 member 2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC23A2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage