Anti-SLC17A5 (Sialin) Antibody | Alomone Labs AST-015 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SLC17A5 (Sialin) Antibody

catalog :

AST-015

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat small intestine (lanes 1 and 3) and brain (lanes 2 and 4) lysates: - 1,2. Anti-SLC17A5 (Sialin) Antibody (#AST-015), (1:200).3,4. Anti-SLC17A5 (Sialin) Antibody, preincubated with SLC17A5/Sialin Blocking Peptide (#BLP-ST015).

image 2 :

Expression of Sialin in rat spinal cord. - Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-SLC17A5 (Sialin) Antibody (#AST-015), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. SLC17A5 immunoreactivity (green) appears in the neuropil of the ventral horn. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AST-015

SKU :

AST-015-CF_0.2 ml

Product Name :

Anti-SLC17A5 (Sialin) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8BN82

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-ST015

Homology :

Rat - 13/14 amino acid residues identical; human - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KGEVQSWALSDHHG, corresponding to amino acid residues 479-492 of mouse SLC17A5

Product Page - Scientific background :

Sialin, encoded by the SLC17A5 gene, is a member of the SLC17 solute carrier family, a group of structurally related polytopic membrane proteins, part of the major facilitator superfamily of transporters. Sialin is a sialic acid transporter located in the lysosomal membrane. In the lysosome, sialic acid residues are sequentially removed from the carbohydrate chain by sialidases and then transported out of the lysosome by the transporter protein sialin1,2. Sialin structure consists of 12 transmembrane helices (TMs). TM4 contains three residues (Arg-168, Glu-175, and His-183) that are highly conserved in the SLC17 family, suggesting that they may participate directly in substrate binding and translocation. In addition, TM4 contains a GXXXG helix packing motif that is important for the expression of sialin. SLC17 family includes several more GXXXG-like motifs located within TMs including GXXXG/S in TM2, G/AXXXG in TM5, and G/S/AXXXGXXXG/T in TM13.Proteins included in the SLC17 family are implicated with inherited neurological and metabolic diseases. Mutations in the SLC17A5 gene cause sialic acid storage diseases including infantile sialic acid storage disease and Salla disease. These conditions are autosomal recessive neurodegenerative disorders characterized by excessive accumulation of sialic acid in the lysosome1,2.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Sialic acid transporter, H+/sialic acid cotransporter, Vesicular H+/aspartate-glutamate cotransporter

Short Description :

A Rabbit Polyclonal Antibody to SLC17A5 (Sialin) Transporter

Long Description :

Anti-SLC17A5 (Sialin) Antibody (#AST-015) is a highly specific antibody directed against an epitope of the mouse Sialin transporter. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Sialin from rat, mouse, and human samples.

Negative Control :

BLP-ST015

Positive Control :

Synonyms :

Sialic acid transporter, H+/sialic acid cotransporter, Vesicular H+/aspartate-glutamate cotransporter

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC17A5

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage