Anti-GPR91 (SUCNR1) (extracellular) Antibody | Alomone Labs ASR-090 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR91 (SUCNR1) (extracellular) Antibody

catalog :

ASR-090

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Centeio R, Ousingsawat J, Schreiber R, Kunzelmann K. CLCA1 Regulates Airway Mucus Production and Ion Secretion Through TMEM16A. Int J Mol Sci. 2021;22: pubmed publisher

image

image 1 :

Western blot analysis of rat kidney membrane (lanes 1 and 3), mouse kidney lysate (lanes 2 and 4), mouse TK-1 T-cell lymphoma (lanes 5 and 8), mouse J774 macrophage cell (lanes 6 and 9) and human Jurkat acute T cell leukemia (lanes 7 and 10) lysates: - 1,2,5,6,7. Anti-GPR91 (SUCNR1) (extracellular) Antibody (#ASR-090) (1:200).3,4,8,9,10. Anti-GPR91 (SUCNR1) (extracellular) Antibody, preincubated with GPR91/SUCNR1 (extracellular) Blocking Peptide (#BLP-SR090).

image 2 :

Expression of GPR91 in mouse hippocampus. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR91 (SUCNR1) (extracellular) Antibody (#ASR-090), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. GPR91 immunoreactivity (green) appears in neuronal profiles in the pyramidal layer (P, vertical arrow) and the stratum oriens (SO, horizontal arrow). B. Pre-incubation of the antibody with GPR91 (SUCNR1) (extracellular) Blocking Peptide (BLP-SR090), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ASR-090

SKU :

ASR-090-CF_0.2 ml

Product Name :

Anti-GPR91 (SUCNR1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q6IYF9

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR090

Homology :

Mouse - 14/15 amino acid residues identical; human - 12/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RNVRIASRLDSWPQG, corresponding to amino acid residues 248-262 of rat GPR91/Succinate Receptor 1

Product Page - Scientific background :

GPR91 (Succinate Receptor 1), a member of the GPCR superfamily, is specifically activated by extracellular succinate outside the tricarboxylic acid cycle, resulting in activation of paracrine and endocrine effectors. Activation of GPR91 is associated with several diseases, including hypertension and diabetes1,2.GPR91 was shown to regulate normal retinal vascularization, proliferative ischemic retinopathy and cortical revascularization post-ischemia. Activation of GPR91 influences different tissues such as kidney, heart, retina, white adipose tissue, and liver.Through the activation of GPR91, succinate affects motility, migration, and growth of dendritic cells. In addition, the receptor directly promotes chemotaxis and potentiates activation initiated by Toll-like receptor agonists in dendritic cells1-3.In the heart GPR91 mediates succinate-induced cardiomyocyte death. It was demonstrated that upholding levels of serum succinate can cause cardiac hypertrophy through the activation of the GPR911,2.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

G-protein coupled receptor 91, Succinate receptor 1

Short Description :

A Rabbit Polyclonal Antibody to Succinate Receptor 1

Long Description :

Anti-GPR91 (SUCNR1) (extracellular) Antibody (#ASR-090) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and live cell flow cytometry. It has been designed to recognize GPR91 from rat, mouse, and human samples.

Negative Control :

BLP-SR090

Positive Control :

Synonyms :

G-protein coupled receptor 91, Succinate receptor 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SUCNR1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage