Anti-GPR91 (SUCNR1) (extracellular)-FITC Antibody | Alomone Labs ASR-090-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR91 (SUCNR1) (extracellular)-FITC Antibody

catalog :

ASR-090-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of GPR91 in live intact human THP-1 monocytic leukemia cells: Cell surface detection of GPR91 in live intact human THP-1 monocytic leukemia cells: Black line: Cells. Red line: Cells + rabbit IgG isotype control-FITC. Green line: Cells + Anti-GPR91 (SUCNR1) (extracellular)-FITC Antibody (#ASR-090-F), (2.5 µg).

product information

CAT :

ASR-090-F

SKU :

ASR-090-F_15 mcl

Product Name :

Anti-GPR91 (SUCNR1) (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q6IYF9

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR090

Homology :

Mouse - 14/15 amino acid residues identical; human - 12/15 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

3rd extracellular loop

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RNVRIASRLDSWPQG, corresponding to amino acid residues 248-262 of rat GPR91/Succinate Receptor 1

Product Page - Scientific background :

GPR91 (Succinate Receptor 1), a member of the GPCR superfamily, is specifically activated by extracellular succinate outside the tricarboxylic acid cycle, resulting in activation of paracrine and endocrine effectors. Activation of GPR91 is associated with several diseases, including hypertension and diabetes1,2.GPR91 was shown to regulate normal retinal vascularization, proliferative ischemic retinopathy and cortical revascularization post-ischemia. Activation of GPR91 influences different tissues such as kidney, heart, retina, white adipose tissue, and liver.Through the activation of GPR91, succinate affects motility, migration, and growth of dendritic cells. In addition, the receptor directly promotes chemotaxis and potentiates activation initiated by Toll-like receptor agonists in dendritic cells1-3.In the heart GPR91 mediates succinate-induced cardiomyocyte death. It was demonstrated that upholding levels of serum succinate can cause cardiac hypertrophy through the activation of the GPR911,2.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

G-protein coupled receptor 91, Succinate receptor 1

Short Description :

A Rabbit Polyclonal Antibody to GPR91 Conjugated to the Fluorescent Dye FITC

Long Description :

Anti-GPR91 (SUCNR1) (extracellular) Antibody (#ASR-090) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and live cell flow cytometry. It has been designed to recognize GPR91 from rat, mouse, and human samples. Anti-GPR91 (SUCNR1) (extracellular)-FITC Antibody (#ASR-090-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

G-protein coupled receptor 91, Succinate receptor 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SUCNR1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ASR-090), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage