Anti-Semaphorin 7A (SEMA7A) (extracellular) Antibody | Alomone Labs ASR-077 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Semaphorin 7A (SEMA7A) (extracellular) Antibody

catalog :

ASR-077

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-Semaphorin 7A (SEMA7A) (extracellular) Antibody (#ASR-077), (1:200).3,4. Anti-Semaphorin 7A (SEMA7A) (extracellular) Antibody, preincubated with Semaphorin 7A/SEMA7A (extracellular) Blocking Peptide (#BLP-SR077).

product information

CAT :

ASR-077

SKU :

ASR-077-CF_0.2 ml

Product Name :

Anti-Semaphorin 7A (SEMA7A) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9QUR8

Applications :

IFC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR077

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RKQEYNGKIPRFRR, corresponding to amino acid residues 190-203 of mouse SEMA7A

Product Page - Scientific background :

The semaphorins are a family of soluble and membrane associated proteins characterized by a conserved "sema" domain. They were originally described as axonal growth and guidance proteins required to guide neuronal axons to the right targets. Today, semaphorins are known to possess pleiotropic and important functions in several physiological and pathogenic processes, including heart development, vascular growth, tumor progression, immune cell regulation, angiogenesis and vasculogenesis1,2.To date more than 20 types of semaphorin proteins have been identified. Semaphorin-7A, also known as CD108, promotes olfactory bulb axon outgrowth in the nervous system. It is also required for the appropriate formation of the lateral olfactory tract during embryonic development1.Some of semaphorin-7A functions are mediated through β1-integrins and the transmembrane protein plexin C1 to which it binds exclusively. Studies show that in the airway remodeling of patients with chronic asthma, semaphorin 7A is a potentially important modulator of eosinophil profibrotic functions. In addition, semaphorin-7A plays an important role as an inducer of pulmonary fibrosis and liver fibrogenesis2.The semaphorin family has been classified into eight subclasses. Unlike other semaphorins, which are integral membrane proteins, semaphorin 7A is anchored to the membrane by a glycophosphatidylinositol (GPI)-linked sequence. The conserved amino-terminal "Sema" domain of the protein includes arginine-glycine-aspartate sequence1.

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

CD108, Semaphorin-K1, Semaphorin-L, JMH blood group antigen

Short Description :

A Rabbit Polyclonal Antibody to Semaphorin 7A

Long Description :

Anti-Semaphorin 7A (SEMA7A) (extracellular) Antibody (#ASR-077) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize SEMA7A from rat, mouse, and human samples.

Negative Control :

BLP-SR077

Positive Control :

Synonyms :

CD108, Semaphorin-K1, Semaphorin-L, JMH blood group antigen

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SEMA7A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage