Anti-Semaphorin 6A (extracellular) Antibody | Alomone Labs ASR-071 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Semaphorin 6A (extracellular) Antibody

catalog :

ASR-071

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1-2. Anti-Semaphorin 6A (extracellular) Antibody (#ASR-071), (1:200). 3-4. Anti-Semaphorin 6A (extracellular) Antibody, preincubated with Semaphorin 6A (extracellular) Blocking Peptide (BLP-SR071).

image 2 :

Expression of Semaphorin 6A in rat cerebellum. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Semaphorin 6A (extracellular) Antibody (#ASR-071), (1:400), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. Semaphorin 6A immunoreactivity (red) appears in the molecular layer (MOL) and in the Purkinje cell layer (P) (arrows). B. Pre-incubation of the antibody with Semaphorin 6A (extracellular) Blocking Peptide (BLP-SR071), suppressed staining. Cell nuclei are stained with DAPI (blue). MOL = molecular layer, P = Purkinje cell layer

product information

CAT :

ASR-071

SKU :

ASR-071-CF_0.2 ml

Product Name :

Anti-Semaphorin 6A (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O35464

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR071

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-term.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)NHQDKKGVIRESYLK, corresponding to amino acid residues 626 - 640 of mouse Semaphorin 6A

Product Page - Scientific background :

Semaphorin 6A (Sema6A) is a member of the semaphorin family of genes involved in cell migration, axon guidance, development of bone1,2, vasculature3, cancer metastasis4, B-cell aggregation and differentiation5, and synaptogenesis6-12, as well as in inhibiting synaptic terminal arborization13.The semaphorins represent one of the largest families of axon guidance cues consisting of eight classes. Members are classified according to structural criteria. All semaphorins share a highly conserved Sema-domain of about 500 amino acids14 with 17 conserved cysteines. Classes 1 and 2 are found only in invertebrates, while classes 3 to 7 contain the vertebrate family members, and class V members are encoded by viral genomes. Semaphorins include both transmembrane (classes 1, 4, 5 and 6), glycosylphosphatidyl inositol (class 7) and secreted (classes 2, 3 and V) proteins15.Initially, all semaphorin family members were believed to induce inhibitory actions on axon pathfinding, branching or targeting, but there is increasing evidence that semaphorins may also have a role in chemoattraction13,16-19. Mutations of Sema6A in mice is associated with a spectrum of subtle defects in cell migration and axon guidance in various brain areas, including the thalamocortical system, hippocampus, cerebellum and various other structures6-12,20. Previous study of gene expression analysis revealed alterations in semaphorin and plexin expression in the prefrontal cortex of patients with schizophrenia20.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

SEMAQ, SEMA6A-1, SEMA6A

Short Description :

A Rabbit Polyclonal antibody to Semaphorin 6A

Long Description :

Anti-Semaphorin 6A (extracellular) Antibody (#ASR-071) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Semaphorin 6A from rat, mouse and human samples.

Negative Control :

BLP-SR071

Positive Control :

Synonyms :

SEMAQ, SEMA6A-1, SEMA6A

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SEMA6A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage