Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody | Alomone Labs ASR-065 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody

catalog :

ASR-065

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

product information

CAT :

ASR-065

SKU :

ASR-065-CF_0.2 ml

Product Name :

Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9H3S1

Applications :

IFC IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR065

Homology :

Mouse, rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KELNKETSRWTTYRG, corresponding to amino acid residues 355 - 369 of human Semaphorin 4A

Product Page - Scientific background :

Neuroimmune Semaphorin 4A (Sema4A) is a member of Semaphorin family of transmembrane and secreted proteins.The Semaphorin protein family members regulate the functional activity of axons in the nervous system, and Sema4A is an important regulator of neuronal and immune functions 1,2. In accordance to that, it was shown to be preferentially expressed on dendritic cells and B cells in the immune system 3.Sema4A consists of an NH2-terminal signal peptide followed by a Sema domain, an Ig domain of the C2 type, a hydrophobic transmembrane region, and a cytoplasmic tail 2, it has seven currently known receptors through which it mediates a complex system of intracellular and extracellular signals that regulate different physiological and pathological tissue processes 1. In the nervous system, Sema4A serves as an axon guidance molecule and in the immune system it regulates immune cell activation and function, instructing the fine tuning of the immune response 1.Recent studies have shown a dysregulation of Sema4A expression in several types of cancer such as hepatocellular carcinoma, colorectal, and breast cancers 1,4. The function of Sema4A in angiogenesis and cancer is not defined but it is shown to be related to the process of angiogenesis. Understanding the role of Sema4A in pathologic processes underlining tumorigenesis and tumor metastasis may guide the development of improved therapeutic treatments for cancer 1.

Applications may also work in :

IFC IHC WB

Supplier :

Alomone Labs

Target :

SEMAB

Short Description :

A Rabbit Polyclonal antibody to Semaphorin 4A (extracellular).

Long Description :

Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody (#ASR-065) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize Semaphorin 4A from mouse, rat and human samples.

Negative Control :

BLP-SR065

Positive Control :

Synonyms :

SEMAB

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SEMA4A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage