Anti-Semaphorin 4D (SEMA4D) (extracellular) Antibody | Alomone Labs ASR-064 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Semaphorin 4D (SEMA4D) (extracellular) Antibody

catalog :

ASR-064

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: - 1,2. Anti-Semaphorin-4D (SEMA4D) (extracellular) Antibody (#ASR-064), (1:200).3,4. Anti-Semaphorin-4D (SEMA4D) (extracellular) Antibody, preincubated with Semaphorin 4D/SEMA4D (extracellular) Blocking Peptide (#BLP-SR064).

product information

CAT :

ASR-064

SKU :

ASR-064-CF_0.2 ml

Product Name :

Anti-Semaphorin 4D (SEMA4D) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O09126

Applications :

IFC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR064

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)EKQHEVYWKVSEDK, corresponding to amino acid residues 80-93 of mouse SEMA4D

Product Page - Scientific background :

Semaphorins are a family of soluble and membrane associated proteins characterized by a conserved "sema" domain. They were originally described as axonal growth and guidance proteins required to guide neuronal axons to the right targets. Today semaphorins are known to possess pleiotropic and important functions in several physiological and pathological processes, including heart development, vascular growth, tumor progression, immune cell regulation, angiogenesis and vasculogenesis. To date, more than 20 types of semaphorin proteins have been identified and classified into eight subclasses1,2. The conserved "sema" domain of the protein includes arginine-glycine-aspartate sequences. The C-terminal region includes an IgG-like domain, a transmembrane domain, and a short cytoplasmic tail that contains one tyrosine phosphorylation site and multiple sites for serine-threonine phosphorylation1,2.Semaphorin 4D (Sema4D), also known as CD100, is a 150-kDa membrane-associated-type class IV semaphorin and was the first semaphorin shown to have immune regulatory activity. Other Sema4D functions include inactivation of platelets, stimulation of angiogenesis, and regulation of bone formation. Sema4D is expressed in many tissues including the brain, kidney, and heart. Sema4D is expressed in resting T cells, and expression is increased upon cellular activation3.

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

CD100, Semaphorin-J

Short Description :

A Rabbit Polyclonal Antibody to Semaphorin 4D

Long Description :

Anti-Semaphorin 4D (SEMA4D) (extracellular) Antibody (#ASR-064) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize SEMA4D from rat, mouse, and human samples.

Negative Control :

BLP-SR064

Positive Control :

Synonyms :

CD100, Semaphorin-J

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SEMA4D

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage