Anti-Semaphorin-3F Antibody | Alomone Labs ASR-056 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Semaphorin-3F Antibody

catalog :

ASR-056

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 5), rat lung membranes (lanes 2 and 6), mouse heart membranes (lanes 3 and 7) and rat dorsal root ganglion lysate (lanes 4 and 8): - 1-4. Anti-Semaphorin-3F Antibody (#ASR-056), (1:400). 5-8. Anti-Semaphorin-3F Antibody, preincubated with Semaphorin-3F Blocking Peptide (#BLP-SR056) (#BLP-SR056).

product information

CAT :

ASR-056

SKU :

ASR-056-CF_0.2 ml

Product Name :

Anti-Semaphorin-3F Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q13275

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR056

Homology :

Mouse, rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Secreted

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)RERSAEAPQSPAVYAR, corresponding to amino acid residues 280 - 295 of human Semaphorin-3F

Product Page - Scientific background :

Semaphorins are a large family of proteins with over 30 members, which are further divided in subgroups1. They are involved in signaling and are implicated in diverse developmental processes such as axon guidance during nervous system development and regulation of cell migration2. They are highly expressed in mammary gland, kidney, fetal brain, and lung and expressed at lower levels in heart and liver tissues2.All semaphorin members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues3. The semaphorin 3 family have a basic domain at the C-terminus. They bind neuropilins which subsequently activate signaling through associated plexins. Semaphorin-3B and semaphorin-3F function additionally as potent inhibitors of tumor development in small cell lung carcinoma. Recent evidence indicates that these semaphorins modulate the adhesive and migratory properties of responsive malignant cells. Semaphorin-3F was also functions as an inhibitor of angiogenesis, contributing significantly to its anti-tumorigenic properties1.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Semaphorin IV, SEMA3F

Short Description :

A Rabbit Polyclonal Antibody to Semaphorin-3F

Long Description :

Anti-Semaphorin-3F Antibody (#ASR-056) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize SEMA3F from mouse, rat, and human samples.

Negative Control :

BLP-SR056

Positive Control :

Synonyms :

Semaphorin IV, SEMA3F

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SEMA3F

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage