Anti-Semaphorin 3A (SEMA3A) Antibody | Alomone Labs ASR-051 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Semaphorin 3A (SEMA3A) Antibody

catalog :

ASR-051

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: - 1,2. Anti-Semaphorin 3A (SEMA3A) Antibody (#ASR-051), (1:400).3,4. Anti-Semaphorin 3A (SEMA3A) Antibody, preincubated with Semaphorin 3A/SEMA3A Blocking Peptide (#BLP-SR051).

image 2 :

Western blot analysis of human THP-1 acute monocytic leukemia cell lysate (lanes 1 and 3) and human MDA-231 breast adenocarcinoma cell lysate (lanes 2 and 4): - 12.Anti-Semaphorin 3A (SEMA3A) Antibody(#ASR-051) (1:400).34. Anti-Semaphorin 3A (SEMA3A) Antibody preincubated with the negative control antigen.

image 3 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4)brain membranes: - 12.Anti-Semaphorin 3A (SEMA3A) Antibody(#ASR-051) (1:400).34. Anti-Semaphorin 3A (SEMA3A) Antibody preincubated with the negative control antigen.

product information

CAT :

ASR-051

SKU :

ASR-051-CF_0.2 ml

Product Name :

Anti-Semaphorin 3A (SEMA3A) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q63548

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR051

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KRDRKQRRQRPGH, corresponding to amino acid residues 728-740 of rat SEMA3A

Product Page - Scientific background :

Semaphorins are members of a large family of proteins controlling a variety of processes in the central nervous system (CNS) during development such as cell migration and axonal growth cone guidance but are also expressed in other tissues. Their common feature is a conserved 500 amino acid "sema" domain. There are currently eight defined classes of semaphorins; classes 2 and 3 are secreted proteins while others are GPI-linked or transmembrane proteins.Semaphorin 3A protein is an elongated disc shaped molecule with dimensions of 60X70X45 angstrom and a molecular weight of 65 kDA. The semaphorin fold is a variation of the β propeller topology with seven blades radially arranged around a central axis. Each blade averages 70 amino acids and contains a four stranded antiparallel sheet. The large size of this protein derives from the secondary structural elements attached to each blade.Two units of Semaphorin 3A create a functional Semaphorin 3A dimer. Neuropilin-1 binds to semaphorin-3A and competes with semaphorin dimerization indicating that the neuropilin-1 binding region of Semaphorin 3A is in proximity to the dimerization site1. Another Semaphorin binding molecule is plexin-1. It does not bind to semaphorin alone but only in a complex with Neuropilin-1. Interestingly, this complex has higher affinity to semaphorin than Neuropilin-1 alone. In addition, binding of neuropilin-1 does not change non-neuronal cell morphology while the combination of Neuropilin-1 and plexin-1 induces an alteration in adherent cells2.The downregulation of Semaphorin expression was found to be linked to many pathological conditions of the CNS such as ischemia, degenerative diseases and multiple sclerosis and it was also found that semaphorin-3A encourages tumor growth in multiple myeloma3.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Semaphorin III, Collapsin 1

Short Description :

A Rabbit Polyclonal Antibody to Semaphorin 3A

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Semaphorin 3A. Anti-Semaphorin 3A (SEMA3A) Antibody (#ASR-051) can be used in western blot analysis. It has been designed to recognize SEMA3A from mouse, rat and human samples.

Negative Control :

BLP-SR051

Positive Control :

Synonyms :

Semaphorin III, Collapsin 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SEMA3A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage