Anti-5HT5B Receptor (HTR5B) (extracellular) Antibody | Alomone Labs ASR-040 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-5HT5B Receptor (HTR5B) (extracellular) Antibody

catalog :

ASR-040

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat and mouse brain lysates: - 1,2. Anti-5HT5B Receptor (HTR5B) (extracellular) Antibody (#ASR-040), (1:400).3,4. Anti-5HT5B Receptor (HTR5B) (extracellular) Antibody, preincubated with 5HT5B Receptor/HTR5B (extracellular) Blocking Peptide (#BLP-SR040).

image 2 :

Western blot analysis of rat and mouse brain lysates: - 12. Anti-5HT5B Receptor (HTR5B) (extracellular) Antibody(#ASR-040) (1:400).34. Anti-5HT5B Receptor (HTR5B) (extracellular) Antibody preincubated with the negative control antigen.

image 3 :

Peptide CSPAPGSWLNLSHVDGN corresponding to amino acid residues 22-38 of rat -Opioid receptor (AccessionP33535). Extracellular N-terminus.

product information

CAT :

ASR-040

SKU :

ASR-040-CF_0.2 ml

Product Name :

Anti-5HT5B Receptor (HTR5B) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P35365

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR040

Homology :

Mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CSDSPSSGRSMG, corresponding to amino acid residues 22-33 of rat 5-Hydroxytryptamine receptor 5B

Product Page - Scientific background :

Serotonin (5-hydroxytryptamine, 5-HT) is a monoamine neurotransmitter synthesized from the amino acid tryptophan by the enzyme tryptophan-decarboxylase. This conversion occurs in chromaffin and enteric cells whereas serotonin reaches the central nervous system via blood circulation. In addition to its function as a neurotransmitter in the CNS, it serves as a precursor for melatonin in the pineal gland. It can also be found in non-neuronal tissues such as the mucosa of gut and blood platelets. Serotonin is known to affect numerous behavioral and physiological functions1.The 5-HT5B receptor is part of family of 13 known G-protein coupled receptors (GPCRs) divided into seven classes based on structural and operational features. Like other GPCRs, 5-HT5B receptor has an extracellular N-terminus, transmembrane domains and an intracellular C-terminal tail2.There are currently two known receptors in the 5-HT5 family. The function of these receptors is far from fully understood and it is unclear with which G-protein they couple. The 5-HT5B gene has been mapped in the human genome but its sequence does not produce a functional gene and no physiological response was found to correspond with its activity3.

Applications may also work in :

Supplier :

Alomone Labs

Target :

5-Hydroxytryptamine receptor 5B, Serotonin receptor 5B, MR22

Short Description :

A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 5B

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat 5-Hydroxytryptamine receptor 5B. Anti-5HT5B Receptor (HTR5B) (extracellular) Antibody (#ASR-040) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize 5HT5B receptor from rat and mouse samples.

Negative Control :

BLP-SR040

Positive Control :

Synonyms :

5-Hydroxytryptamine receptor 5B, Serotonin receptor 5B, MR22

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HTR5BP

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage