Anti-5HT7 Receptor/HTR7 (extracellular) Antibody | Alomone Labs ASR-037 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-5HT7 Receptor/HTR7 (extracellular) Antibody

catalog :

ASR-037

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): - 1, 2. Anti-5HT7 Receptor/HTR7 (extracellular) Antibody (#ASR-037), (1:400). 3, 4. Anti-5HT7 Receptor/HTR7 (extracellular) Antibody, preincubated with 5HT7 Receptor/HTR7 (extracellular) Blocking Peptide (#BLP-SR037).

image 2 :

Expression of 5HT7 Receptor in rat dorsal raphe nucleus (DRN) - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-5HT7 Receptor/HTR7 (extracellular) Antibody (#ASR-037), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. 5HT7R immunoreactivity (green) appears in neuronal profiles in the lateral DRN (arrows). B. Pre-incubation of the antibody with 5HT7 Receptor/HTR7 (extracellular) Blocking Peptide (#BLP-SR037), suppresses staining. Cell nuclei are stained with DAPI (blue). LDRN = lateral DRN, MDRN = medial DRN.

product information

CAT :

ASR-037

SKU :

ASR-037-CF_0.2 ml

Product Name :

Anti-5HT7 Receptor/HTR7 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P32305

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR037

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CGEQINYGRVEK, corresponding to amino acid residues 73 - 84 of rat 5-HT7

Product Page - Scientific background :

5-HT7 receptor is a G-protein coupled receptor (GPCR) that is activated by Serotonin. Like all other members, 5-HT7 has seven transmembrane domains. In addition, it contains a hydrophobic domain located at its N-terminal end1.The signaling cascades activated by these receptors take part in circadian rhythm, learning and memory, hippocampal signaling and smooth muscle relaxation. It was also shown to have a role in several disorders including Autism, neuropsychiatric disorders, Epilepsy and Alzheimer's disease2,4.5-HT7 is encoded by HTR7 gene and it has three splice variants encoding proteins that differ in the length of their carboxy-terminal ends. The splice variants are differentially expressed in the spleen, kidney, heart, thalamus, hindbrain, hippocampus, cortex, caudate, striatum and cerebellum3.Due to its involvement in central and the peripheral nervous systems, endocrine system and its involvement in various diseases and syndromes, 5-HT7 receptor represents an interesting target for the treatment and prevention of various pathologies. Selective ligands are being studied in order to regulate this receptor's activity4.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

5-Hydroxytryptamine Receptor 7, Serotonin Receptor 7, 5-HT7, 5-HT-7

Short Description :

A Rabbit Polyclonal Antibody to 5HT7 Receptor

Long Description :

Anti-5HT7 Receptor/HTR7 (extracellular) Antibody (#ASR-037) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize 5HT7 from human, rat, and mouse samples.

Negative Control :

BLP-SR037

Positive Control :

Synonyms :

5-Hydroxytryptamine Receptor 7, Serotonin Receptor 7, 5-HT7, 5-HT-7

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HTR7

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage