Anti-5HT4 Receptor (HTR4) (extracellular) Antibody | Alomone Labs ASR-036 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-5HT4 Receptor (HTR4) (extracellular) Antibody

catalog :

ASR-036

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Salaga M, Binienda A, Piscitelli F, Mokrowiecka A, Cygankiewicz A, Verde R, et al. Systemic administration of serotonin exacerbates abdominal pain and colitis via interaction with the endocannabinoid system. Biochem Pharmacol. 2019;161:37-51 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Anti-5HT4 Receptor (HTR4) (extracellular) Antibody (#ASR-036), (1:200).2. Anti-5HT4 Receptor (HTR4) (extracellular) Antibody, preincubated with 5HT4 Receptor/HTR4 (extracellular) Blocking Peptide (#BLP-SR036).

image 2 :

Peptide (C)DVIEKRKFNHNSN corresponding to amino acid residues 168-180 of rat 5-Hydroxytryptamine receptor 4 (AccessionQ62758). 2ndextracellular loop.

image 3 :

Expression of Serotonin receptor 4 in rat PC12 cells - Cell surface detection ofSerotonin receptor 4 in intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-5HT4 Receptor (HTR4) (extracellular) Antibody (#ASR-036) (1:100) (red). B. Merge of A with a live view of the cell.

product information

CAT :

ASR-036

SKU :

ASR-036-CF_0.2 ml

Product Name :

Anti-5HT4 Receptor (HTR4) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q62758

Applications :

IC IFC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR036

Homology :

Mouse - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DVIEKRKFNHNSN, corresponding to amino acid residues 168-180 of rat 5-Hydroxytryptamine receptor 4

Product Page - Scientific background :

5-hydroxytryptamine receptor 4 (5-HT4 receptors) are a subclass among the large family of receptors mediating serotonin actions at central and peripheral targets. 5-HT4 receptor activation has been implicated in learning and memory, feeding control and stress response. 5-HT4 receptors are encoded by a complex gene which in humans, generates ten different splice variants1, 2.The 5-HT4 receptor, a member of the seven transmembrane spanning G-protein-coupled receptors (GPCRs), couples via Gs to increase cAMP production. GPCRs share a similar topology, with seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs); the N-terminus is on the extracellular side of the membrane, and the C-terminus is on the cytoplasmic side3.Within the central nervous system, these receptors are expressed in limbic structures including: hypothalamus, nucleus accumbens, prefrontal cortex, amygdala and hippocampus, where they contribute to emotional and cognitive functions4.Low levels of 5-HT4 receptors are associated with numerous neuropsychiatric disorders including Alzheimer's disease, Huntington's disease, feeding disorders and stress-related disorders such as anxiety and depression5.

Applications may also work in :

IC IFC LCI WB

Supplier :

Alomone Labs

Target :

5-Hydroxytryptamine receptor 4, Serotonin receptor 4

Short Description :

A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 4

Long Description :

Anti-5HT4 Receptor (HTR4) (extracellular) Antibody (#ASR-036) is a highly specific antibody directed against an epitope of the rat Serotonin receptor 4. The antibody can be used in western blot, immunocytochemistry, and live cell imaging applications. It has been designed to recognize 5HT4 receptor from rat, mouse, and human samples.

Negative Control :

BLP-SR036

Positive Control :

Synonyms :

5-Hydroxytryptamine receptor 4, Serotonin receptor 4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HTR4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage